ArticleScientific reports2018
Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Trial
- Maternal exposure to cyclophosphamide during prepubertal life does not affect the cryosusceptibility of in vitro derived mouse embryos.Reproduction & fertility · 2026Article
- Oocyte and embryo developmental competence following small multiple cyclophosphamide dose administrations in prepubertal female mice are comparable to adolescents.Scientific reports · 2025Article
- New approaches in the analysis of spent embryo culture media in the IVF process.Archives of gynecology and obstetrics · 2025Article
- Advanced maternal age affects their frozen-thawed embryo susceptibility to high oxygen environment.Scientific reports · 2024Article
- Embryos from Prepubertal Hyperglycemic Female Mice Respond Differentially to Oxygen Tension In Vitro.Cells · 2024Article
- DNA damage in preimplantation embryos and gametes: specification, clinical relevance and repair strategies.Human reproduction update · 2022Review
- Clinical Relevance of Secreted Small Noncoding RNAs in an Embryo Implantation Potential Prediction at Morula and Blastocyst Development Stages.Life (Basel, Switzerland) · 2021Article
- Review
- Review
- Advantages of the outgrowth model for evaluating the implantation competence of blastocysts.Clinical and experimental reproductive medicine · 2020Review
- Article
- Embryo competence and cryosurvival: Molecular and cellular features.Animal reproduction · 2019Article
- Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The metabolomic profile of an embryo culture medium can aid in the advanced prediction of embryonic developmental potential and genetic integrity. But it is not known if this technology can be used to determine the in vitro potential of inner cell mass (ICM) in adherence and proliferation. Here, we investigated the developmental potential of mouse 2-cell embryos carrying cisplatin-induced DNA lesions (IDL), beyond blastocyst stage using ICM outgrowth assay. The genetic integrity of ICM cells was determined by comet assay. The metabolic signatures of spent medium were recorded 84 hours post injection of hCG (hpi-hCG), and after 96 hours of extended in vitro culture (Ex 96) by NMR spectroscopy. We observed that blastocysts that lack the ability to adhere in vitro had an increased requirement of pyruvate (p < 0.01), lactate (p < 0.01), and were accompanied by a significant reduction of pyruvate-alanine ratio in the culture medium. We propose that the aforementioned metabolites from 84 hpi-hCG spent medium be further explored using appropriate experimental models, to prove their potential as biomarkers in the prediction of implantation ability of in vitro derived human embryos in clinical settings.
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