ArticlePlant biotechnology journal2019
Isolation of amaranthin synthetase from Chenopodium quinoa and construction of an amaranthin production system using suspension-cultured tobacco BY-2 cells.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- A cyclo-DOPA 6-O-glucosyltransferase-mediated route for gomphrenin I biosynthesis in Basella alba and Gomphrena globosa.The New phytologist · 2026Article
- Searching for Amaranthin-A Multipotential Betacyanin from Natural Sources and In Vitro Cultures.International journal of molecular sciences · 2026Review
- Recombinant production of amaranthin and other betalain variants with yeast cell factories.Synthetic and systems biotechnology · 2025Article
- Metabolically engineered plant cell cultures as biofactories for the production of high-value carotenoids astaxanthin and canthaxanthin.Scientific reports · 2025Article
- Identification ofPlant biotechnology (Tokyo, Japan) · 2025Article
- Screening of Plant UDP-Glycosyltransferases for Betanin Production in Yeast.Applied biochemistry and biotechnology · 2025Article
- BAHD acyltransferase from dragon fruit enables production of phyllocactin in engineered yeast.FEMS yeast research · 2025Article
- Exploring the hidden treasure in arid regions: pseudocereals as sustainable, climate-resilient crops for food security.Frontiers in plant science · 2025Review
- Chromosome-level genome assemblies for two quinoa inbred lines from northern and southern highlands of Altiplano where quinoa originated.Frontiers in plant science · 2024Article
- Identification, expression analysis of quinoa betalain biosynthesis genes and their role in seed germination and cold stress.Plant signaling & behavior · 2023Article
- Engineering a complex, multiple enzyme-mediated synthesis of natural plant pigments in the silkworm,Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genomic Variation Underlying the Breeding Selection of Quinoa Varieties Longli-4 and CA3-1 in China.International journal of molecular sciences · 2022Article
- Red-Beet Betalain Pigments Inhibit Amyloid-β Aggregation and Toxicity in Amyloid-β Expressing Caenorhabditis elegans.Plant foods for human nutrition (Dordrecht, Netherlands) · 2022Article
- A chromosome-scale genome sequence of pitaya (Hylocereus undatus) provides novel insights into the genome evolution and regulation of betalain biosynthesis.Horticulture research · 2021Article
- Virus-Mediated Transient Expression Techniques Enable Functional Genomics Studies and Modulations of Betalain Biosynthesis and Plant Height in Quinoa.Frontiers in plant science · 2021Article
- A novel WD40-repeat protein involved in formation of epidermal bladder cells in the halophyte quinoa.Communications biology · 2020Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Betalains are plant pigments primarily produced by plants of the order Caryophyllales. Because betalain possesses anti-inflammatory and anticancer activities, it may be useful as a pharmaceutical agent and dietary supplement. Recent studies have identified the genes involved in the betalain biosynthesis of betanin. Amaranthin and celosianin II are abundant in the quinoa (Chenopodium quinoa Willd.) hypocotyl, and amaranthin comprises glucuronic acid bound to betanin; therefore, this suggests the existence of a glucuronyltransferase involved in the synthesis of amaranthin in the quinoa hypocotyl. To identify the gene involved in amaranthin biosynthesis, we performed a BLAST analysis and phylogenetic tree analysis based on sequences homologous to flavonoid glycosyltransferase, followed by expression analysis on the quinoa hypocotyl to obtain three candidate proteins. Production of amaranthin in a transient Nicotiana benthamiana expression system was evaluated for these candidates and one was identified as having the ability to produce amaranthin. The gene encoding this protein was quinoa amaranthin synthetase 1 (CqAmaSy1). We also created a transgenic tobacco bright yellow-2 (BY-2) cell line wherein four betalain biosynthesis genes were introduced to facilitate amaranthin production. This transgenic cell line produced 13.67 ± 4.13 μm (mean ± SEM) amaranthin and 26.60 ± 1.53 μm betanin, whereas the production of isoamaranthin and isobetanin could not be detected. Tests confirmed the ability of amaranthin and betanin to slightly suppress cancer cell viability. Furthermore, amaranthin was shown to significantly inhibit HIV-1 protease activity, whereas betanin did not.
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