Article in Biotechnology and bioengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literature
Who cites it
1 citing paper in PubMed, 1 citations in OpenAlex.
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what money
Authors and funding
10 authors at 3 institutions in 1 country.
C Seth PearsonHoward P Isermann Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York.
Reza NematiDepartment of Chemistry, State University of New York, Albany, New York.
Binbin LiuLaboratory of Computational & Structural Biology, Wadsworth Center, New York State Department of Health, Albany, New York.
Jing ZhangLaboratory of Computational & Structural Biology, Wadsworth Center, New York State Department of Health, Albany, New York.
Matteo ScalabrinDepartment of Chemistry, State University of New York, Albany, New York.
Zhong LiLaboratory of Computational & Structural Biology, Wadsworth Center, New York State Department of Health, Albany, New York.
Hongmin LiLaboratory of Computational & Structural Biology, Wadsworth Center, New York State Department of Health, Albany, New York.ORCID 0000-0002-8684-5308
Dan FabrisDepartment of Chemistry, State University of New York, Albany, New York.
Marlene BelfortDepartment of Biomedical Sciences, School of Public Health, Albany, State University of New York, Albany, New York.ORCID 0000-0002-1592-5618
Georges BelfortHoward P Isermann Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York.ORCID 0000-0002-7314-422X
Albany State University · USNew York State Department of Health · USRensselaer Polytechnic Institute · US
Funding
SELF-SPLICING INTEINS: FUNCTION, EVOLUTION, APPLICATIONR01GM044844 · NIGMS · WADSWORTH CENTER · PI BELFORT, MARLENE · 1990 to 2019
$8.9M
SELF-SPLICING INTRONS IN THE PHAGE T4/E COLI SYSTEMR01GM039422 · NIGMS · WADSWORTH CENTER · PI BELFORT, MARLENE · 1988 to 2019
$5.3M
Modular and Project Based Training to Enhance Rigor and Reproducibility in Research for Diverse Biomedical CareersT32GM067545 · NIGMS · RENSSELAER POLYTECHNIC INSTITUTE · PI VASHISHTH, DEEPAK · 2004 to 2020
$3.0M
Characterization of ncRNAs' post-transcriptional modifications by antisense affinity capture and MS analysisR01GM121844 · NIGMS · UNIVERSITY OF CONNECTICUT STORRS · PI FABRIS, DANIELE · 2017 to 2021
$1.6M
Role of Post-transcription RNA Modifications on Zika Virus Gene ExpressionR21AI133617 · NIAID · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI FABRIS, DANIELE, PAGER, CARA THERESIA · 2017 to 2018
We have engineered an intein which spontaneously and reversibly forms a thiazoline ring at the native N-terminal Lys-Cys splice junction. We identified conditions to stablize the thiazoline ring and provided the first crystallographic evidence, at 1.54 Å resolution, for its existence at an intein active site. The finding bolsters evidence for a tetrahedral oxythiazolidine splicing intermediate. In addition, the pivotal mutation maps to a highly conserved B-block threonine, which is now seen to play a causative role not only in ground-state destabilization of the scissile N-terminal peptide bond, but also in steering the tetrahedral intermediate toward thioester formation, giving new insight into the splicing mechanism. We demonstrated the stability of the thiazoline ring at neutral pH as well as sensitivity to hydrolytic ring opening under acidic conditions. A pH cycling strategy to control N-terminal cleavage is proposed, which may be of interest for biotechnological applications requiring a splicing activity switch, such as for protein recovery in bioprocessing.
Indexed as
InteinsBacterial ProteinsCrystallography, X-RayHumansModels, MolecularMutationMycobacterium tuberculosisProtein ConformationProtein SplicingRec A RecombinasesThiazolesTuberculosisBacterial ProteinsRec A RecombinasesThiazolescleavage controlmolecular switchprotein splicingthiazoline crystal structure
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Structure of an engineered intein reveals thiazoline ring and provides mechanistic insight. · full record | OpenQuestion