ArticleMicrobiologyOpen2019
Establishment of a highly efficient conjugation protocol for Streptomyces kanamyceticus ATCC12853.
Article in MicrobiologyOpen, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Fluorescence-Based Soil Survival Analysis of the Xenobiotic- and Metal-DetoxifyingInternational journal of molecular sciences · 2025Article
- Enhancing conjugation from E. coli to Streptomyces coelicolor by incorporating traJ into mobilizable plasmids.Applied microbiology and biotechnology · 2025Article
- Improvement of the conjugation transfer of N. gerenzanensis based on the synergistic effect of quorum sensing and antibiotic interference.AMB Express · 2023Article
- Enabling Efficient Genetic Manipulations in a Rare ActinomyceteFrontiers in microbiology · 2022Article
- Trans-Kingdom Conjugation within Solid Media fromInternational journal of molecular sciences · 2019Article
- Establishment of a highly efficient conjugation protocol for Streptomyces kanamyceticus ATCC12853.MicrobiologyOpen · 2019Article
- Development and optimization of an intergeneric conjugation system and analysis of promoter activity in Streptomyces rimosus M527.Journal of Zhejiang University. Science. BArticle
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kanamycin B as the secondary metabolite of wild-type Streptomyces kanamyceticus (S. kanamyceticus) ATCC12853 is often used for the synthesis of dibekacin and arbekacin. To construct the strain has the ability for kanamycin B production; the pSET152 derivatives from Escherichia coli ET12567 were introduced to S. kanamyceticus by intergeneric conjugal transfer. In this study, we established a reliable genetic manipulation system for S. kanamyceticus. The key factors of conjugal transfer were evaluated, including donor-to-recipient ratio, heat-shock, and the overlaying time of antibiotics. When spores were used as recipient, the optimal conjugation frequency was up to 6.7 × 10
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