ArticleMolecular ecology resources2019
Nonrandom RNAseq gene expression associated with RNAlater and flash freezing storage methods.
Article in Molecular ecology resources, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
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- Optimized methods for obtaining sequencing-quality RNA from blubber of free-ranging cetaceans collected under field conditions.Conservation physiology · 2026Article
- Single-Cell Sequencing Unravels Pancreatic Cancer: Novel Technologies Reveal Novel Aspects of Cellular Heterogeneity and Inform Therapeutic Strategies.Biomedicines · 2025Review
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- Enhanced RNA Preservation in Mouse Brain Tissue: A Strategy Combining Cardiac Perfusion with Hypersaline Immersion.ACS omega · 2025Article
- Experimental reporting of fish transcriptomic responses in environmental toxicology and ecotoxicology.Environmental toxicology and chemistry · 2025Review
- Formalin-fixed paraffin-embedded (FFPE) samples help to investigate transcriptomic responses in wildlife disease.Molecular ecology resources · 2025Article
- RNA quality and protamine gene expression after storage of mouse testes under different conditions.PloS one · 2024Article
- Clearing the air: unraveling past and guiding future research in atmospheric chemosynthesis.Microbiology and molecular biology reviews : MMBR · 2023Review
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- Transcriptome analysis revealed enrichment pathways and regulation of gene expression associated with somatic embryogenesis in Camellia sinensis.Scientific reports · 2023Article
- Test of the FlashFREEZE unit in tissue samples freezing for biobanking purposes.Cell and tissue banking · 2023Article
- Improvements and challenges of tissue preparation for spatial transcriptome analysis of skull base tumors.Heliyon · 2023Article
- Research perspectives-Pipelines to human tendon transcriptomics.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Review
- Non-Lethal Sampling Supports Integrative Movement Research in Freshwater Fish.Frontiers in genetics · 2022Review
- Transcriptome and Metabolome Analysis Revealed the Freezing Resistance Mechanism in 60-Year-Old OverwinteringBiology · 2021Article
- Life-On-Hold: Lanthanoids Rapidly Induce a Reversible Ametabolic State in Mammalian Cells.Biology · 2021Article
- Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing.BMC genomics · 2021Article
- Dark world rises: The emergence of cavefish as a model for the study of evolution, development, behavior, and disease.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
RNA sequencing is a popular next-generation sequencing technique for assaying genome-wide gene expression profiles. Nonetheless, it is susceptible to biases that are introduced by sample handling prior gene expression measurements. Two of the most common methods for preserving samples in both field-based and laboratory conditions are submersion in RNAlater and flash freezing in liquid nitrogen. Flash freezing in liquid nitrogen can be impractical, particularly for field collections. RNAlater is a solution for stabilizing tissue for longer-term storage as it rapidly permeates tissue to protect cellular RNA. In this study, we assessed genome-wide expression patterns in 30-day-old fry collected from the same brood at the same time point that were flash-frozen in liquid nitrogen and stored at -80°C or submerged and stored in RNAlater at room temperature, simulating conditions of fieldwork. We show that sample storage is a significant factor influencing observed differential gene expression. In particular, genes with elevated GC content exhibit higher observed expression levels in liquid nitrogen flash-freezing relative to RNAlater storage. Further, genes with higher expression in RNAlater relative to liquid nitrogen experience disproportionate enrichment for functional categories, many of which are involved in RNA processing. This suggests that RNAlater may elicit a physiological response that has the potential to bias biological interpretations of expression studies. The biases introduced to observed gene expression arising from mimicking many field-based studies are substantial and should not be ignored.
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Registered trials
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