Evidence map›Paper›PMID 30446563›Full record

ArticleApplied and environmental microbiology2019

Noninvasive Detection of Equid Herpesviruses in Fecal Samples.

Peter A Seeber, Anisha Dayaram, Florian Sicks, Nikolaus Osterrieder, Mathias Franz, Alex D Greenwood

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Molecular prevalence of equine alphaherpesvirus-1 shedding in healthy broodmares in Ontario.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Peter A SeeberDepartment of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.ORCID 0000-0003-4401-8248
Anisha DayaramDepartment of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
Florian SicksTierpark Berlin, Berlin, Germany.
Nikolaus OsterriederInstitut für Virologie, Zentrum für Infektionsmedizin, Freie Universität Berlin, Berlin, Germany.
Mathias Franz *Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.ORCID 0000-0001-5111-6503
Alex D Greenwood *Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany greenwood@izw-berlin.ORCID 0000-0002-8249-1565

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Equid herpesviruses (EHVs) are pathogens of equid and nonequid hosts that can cause disease and fatalities in captivity and in the wild. EHVs establish latent infections but can reactivate, and most EHVs are shed via the nasal passage. Therefore, nasal swabs are generally used for EHV monitoring. However, invasive sampling of wild equids is difficult. While feces is a commonly used substrate for detecting other pathogens, to our knowledge, EHVs have never been detected in feces of naturally infected equids. We systematically tested zebra feces for EHV presence by (i) establishing nested PCR conditions for fecal DNA extracts, (ii) controlling for environmental EHV contamination, and (iii) large-scale testing on a free-ranging zebra population. A dilution minimizing inhibition while maximizing viral DNA concentrations was determined in captive Grévy's zebra (

Indexed as

AnimalsAnimals, WildEquidaeFecesGenome, ViralHerpesviridaeHerpesviridae InfectionsReal-Time Polymerase Chain ReactionEquus grevyiEquus quaggaEquus zebraindirect samplingviral DNAwildlife disease monitoring

Identifiers

PMID30446563
PMCPMC6344631

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.