ArticleFertility and sterility2018
Metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes.
Article in Fertility and sterility, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05332769 (Cumulus Cell Mitochondrial Activity as a Non-invasive Marker of Embryo Quality), which is not on this map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Cumulus Cell Mitochondrial Activity as a Non-invasive Marker of Embryo Quality
Who cites it
28 citing papers in PubMed, 59 citations in OpenAlex.
- Metabolic imaging for gamete and embryo assessment through advanced microscopy technologies: a novel avenue for artificial intelligence?Human reproduction (Oxford, England) · 2026Review
- From Static to Dynamic: Fluorescence Imaging Technology Advances Precise Embryo Evaluation.Small methods · 2026Review
- FLIM Characterization of Staurosporine-Induced Changes in Endogenous Cellular Autofluorescence.Chemical & biomedical imaging · 2026Article
- Investigating metabolic activity during oocyte and early embryo development through label-free metabolic imaging: a systematic approach for timelapse applications.Human reproduction (Oxford, England) · 2025Article
- Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.Journal of biophotonics · 2025Review
- Innovations in assisted reproductive technology through the advances in artificial intelligence and image processing.Obstetrics & gynecology science · 2025Review
- HBimmCue: A Versatile Fluorescent Probe for Multi-Scale Imaging of Lipid Polarity and Membrane Order in Inner Mitochondrial Membrane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Mechanistic Study of Mitochondrial Autophagy and Ferroptosis in the Progression of Decreased Ovarian Reserve.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Advanced strategies for single embryo selection in assisted human reproduction: A review of clinical practice and research methods.Clinical and experimental reproductive medicine · 2025Article
- Efficacy Evaluation of "Enhanced" Natural Killers withSovremennye tekhnologii v meditsine · 2025Article
- Novel application of metabolic imaging of early embryos using a light-sheet on-a-chip device: a proof-of-concept study.Human reproduction (Oxford, England) · 2025Article
- A Fresh Look at Islet Isolation from Rabbit Pancreases.International journal of molecular sciences · 2024Article
- METAPHOR: Metabolic evaluation through phasor-based hyperspectral imaging and organelle recognition for mouse blastocysts and oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Ex ovo omnia-why don't we know more about egg quality via imaging?Biology of reproduction · 2024Review
- Metabolic imaging of human cumulus cells reveals associations with pregnancy and live birth.Human reproduction (Oxford, England) · 2024Observational
- Visualizing subcellular changes in the NAD(H) pool size versus redox state using fluorescence lifetime imaging microscopy of NADH.Communications biology · 2024Article
- Comparison of Retinal Metabolic Activity and Structural Development between rd10 Mice and Normal Mice Using Multiphoton Fluorescence Lifetime Imaging Microscopy.Current issues in molecular biology · 2024Article
- Noninvasive metabolic profiling of cumulus cells, oocytes, and embryos via fluorescence lifetime imaging microscopy: a mini-review.Human reproduction (Oxford, England) · 2023Review
- Looking with new eyes: advanced microscopy and artificial intelligence in reproductive medicine.Journal of assisted reproduction and genetics · 2023Review
- Contrast-Free FLIM Reveals Metabolic Changes in Pathological Islets of Langerhans.International journal of molecular sciences · 2022Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveTo determine whether metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) identifies metabolic differences between normal oocytes and those with metabolic dysfunction.
designExperimental study.
settingAcademic research laboratories. PATIENT(S): None. INTERVENTION(S): Oocytes from mice with global knockout of Clpp (caseinolytic peptidase P; n = 52) were compared with wild-type (WT) oocytes (n = 55) as a model of severe oocyte dysfunction. Oocytes from old mice (1 year old; n = 29) were compared with oocytes from young mice (12 weeks old; n = 35) as a model of mild oocyte dysfunction. MAIN OUTCOME MEASURE(S): FLIM was used to measure the naturally occurring nicotinamide adenine dinucleotide dehydrogenase (NADH) and flavin adenine dinucleotide (FAD) autofluorescence in individual oocytes. Eight metabolic parameters were obtained from each measurement (4 per fluorophore): short (τ RESULT(S): In Clpp-knockout oocytes compared with WT, FAD τ CONCLUSION(S): FLIM parameters exhibit strong differentiation between Clpp-knockout versus WT, and old versus young oocytes. FLIM could potentially be used as a noninvasive tool to assess mitochondrial function in oocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.