ArticleEpigenetics & chromatin2018
High-resolution mapping of centromeric protein association using APEX-chromatin fibers.
Article in Epigenetics & chromatin, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Centromere-size reduction and chromatin state dynamics following intergenomic hybridization in cotton.PLoS genetics · 2025Article
- Independence of centromeric and pericentromeric chromatin stability on CCAN components.Molecular biology of the cell · 2025Article
- High-resolution analysis of human centromeric chromatin.Life science alliance · 2025Article
- Argonaute protein CSR-1 restricts localization of holocentromere protein HCP-3, the C. elegans CENP-A homolog.Journal of cell science · 2024Article
- Vertebrate centromere architecture: from chromatin threads to functional structures.Chromosoma · 2024Review
- Efficient formation of single-copy human artificial chromosomes.Science (New York, N.Y.) · 2024Article
- Article
- MLL methyltransferases regulate H3K4 methylation to ensure CENP-A assembly at human centromeres.PLoS biology · 2023Article
- LncRNA CCTT-mediated RNA-DNA and RNA-protein interactions facilitate the recruitment of CENP-C to centromeric DNA during kinetochore assembly.Molecular cell · 2022Article
- Centromere Identity and the Regulation of Chromosome Segregation.Frontiers in cell and developmental biology · 2022Review
- Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome).Molecules (Basel, Switzerland) · 2021Review
- Disruption of the MSL complex inhibits tumour maintenance by exacerbating chromosomal instability.Nature cell biology · 2021Article
- Article
- A genetic memory initiates the epigenetic loop necessary to preserve centromere position.The EMBO journal · 2020Article
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe centromere is a specialized chromosomal locus that forms the basis for the assembly of a multi-protein complex, the kinetochore and ensures faithful chromosome segregation during every cell division. The repetitive nature of the underlying centromeric sequence represents a major obstacle for high-resolution mapping of protein binding using methods that rely on annotated genomes. Here, we present a novel microscopy-based approach called "APEX-chromatin fibers" for localizing protein binding over the repetitive centromeric sequences at kilobase resolution.
resultsBy fusing centromere factors of interest to ascorbate peroxidase, we were able to label their binding profiles on extended chromatin fibers with biotin marks. We applied APEX-chromatin fibers to at least one member of each CCAN complex, most of which show a localization pattern different from CENP-A but within the CENP-A delineated centromeric domain. Interestingly, we describe here a novel characteristic of CENP-I and CENP-B that display extended localization beyond the CENP-A boundaries.
conclusionsOur approach was successfully applied for mapping protein association over centromeric chromatin, revealing previously undescribed localization patterns. In this study, we focused on centromeric factors, but we believe that this approach could be useful for mapping protein binding patterns in other repetitive regions.
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