ArticleExperimental eye research2019
Effect of diabetes and hyaluronidase on the retinal endothelial glycocalyx in mice.
Article in Experimental eye research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 30 citations in OpenAlex.
- The glomerular endothelial glycocalyx as a therapeutic target in proteinuric kidney disease.Nature reviews. Nephrology · 2026Review
- Dysregulated hyaluronan metabolism drives inflammation and angiogenesis in proliferative diabetic retinopathy.Frontiers in immunology · 2026Article
- Western diet-induced endothelial glycocalyx remodeling is partially reversed by fasting.American journal of physiology. Heart and circulatory physiology · 2025Article
- Time course and contributors to greater glycocalyx thickness and integrity following Western diet consumption.American journal of physiology. Heart and circulatory physiology · 2025Article
- Methamphetamine-Induced Loss of Syndecan-1 and Retinal Endothelial Integrity via the TAAR-1/MMP-9 Pathway.Pathophysiology : the official journal of the International Society for Pathophysiology · 2025Article
- Microvascular destabilization and intricated network of the cytokines in diabetic retinopathy: from the perspective of cellular and molecular components.Cell & bioscience · 2024Review
- High Glucose Induces Oxidative Stress That Alters Glycocalyx Proteoglycan Levels in Primary Rat Retinal Microvascular Endothelial Cells and in Isolated Ophthalmic Arteries.Pathophysiology : the official journal of the International Society for Pathophysiology · 2024Article
- Early diabetic kidney disease: Focus on the glycocalyx.World journal of diabetes · 2023Review
- Endothelial glycocalyx in retina, hyperglycemia, and diabetic retinopathy.American journal of physiology. Cell physiology · 2023Review
- The Endothelial Glycocalyx and Retinal Hemodynamics.Pathophysiology : the official journal of the International Society for Pathophysiology · 2022Article
- Effect of high glucose on glycosaminoglycans in cultured retinal endothelial cells and rat retina.Glycobiology · 2022Article
- Damage-Associated Molecular Patterns (DAMPs) in Retinal Disorders.International journal of molecular sciences · 2022Review
- Heparanase as active player in endothelial glycocalyx remodeling.Matrix biology plus · 2022Article
- Glycocalyx is critical for blood-brain barrier integrity by suppressing caveolin1-dependent endothelial transcytosis following ischemic stroke.Brain pathology (Zurich, Switzerland) · 2022Article
- Hyperglycemia-induced effects on glycocalyx components in the retina.Experimental eye research · 2021Article
- Article
- Integrative Biology of Diabetic Retinal Disease: Lessons from Diabetic Kidney Disease.Journal of clinical medicine · 2021Review
- The Glycocalyx and Its Role in Vascular Physiology and Vascular Related Diseases.Cardiovascular engineering and technology · 2021Review
- Update on the Role of the Endothelial Glycocalyx in Angiogenesis and Vascular Inflammation.Frontiers in cell and developmental biology · 2021Review
- Hydrogen Sulfide Is a Novel Protector of the Retinal Glycocalyx and Endothelial Permeability Barrier.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We sought to investigate the effects of diabetes and hyaluronidase on the thickness of the endothelial glycocalyx layer in the mouse retina. In our study, the retinal circulation of diabetic Ins2(Akita) mice and their nondiabetic littermates were observed via intravital microscopy. The endothelial glycocalyx thickness was determined from the infusion of two fluorescently labeled plasma markers, one of which was a high molecular weight rhodamine dextran (MW = 155,000) excluded from the glycocalyx, and the other a more permeable low molecular weight sodium fluorescein (MW = 376). In nondiabetic C57BL/6 mice, the glycocalyx thickness also was evaluated prior to and following infusion of hyaluronidase, an enzyme that can degrade hyaluronic acid on the endothelial surface. A leakage index was used to evaluate the influence of hyaluronidase on the transport of the fluorescent tracers from the plasma into the surrounding tissue, and plasma samples were obtained to measure levels of circulating hyaluronic acid. Both diabetes and hyaluronidase infusion significantly reduced the thickness of the glycocalyx in retinal arterioles (but not in venules), and hyaluronidase increased retinal microvascular leakage of both fluorescent tracers into the surrounding tissue. However, only hyaluronidase infusion (not diabetes) increased circulating plasma levels of hyaluronic acid. In summary, our findings demonstrate that diabetes and hyaluronidase reduce the thickness of the retinal endothelial glycocalyx, in which hyaluronic acid may play a significant role in barrier function.
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