ArticlePLoS biology2018
Identification of molecular determinants that govern distinct STIM2 activation dynamics.
Article in PLoS biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- STIM-Orai signaling in neurons: integrating calcium dynamics and synaptic plasticity.Cell communication and signaling : CCS · 2026Review
- The nonexcitable smooth muscle: remodeling the smooth muscle ion transport toolkit in disease.Physiological reviews · 2026Review
- Glycosphingolipids regulate phosphatidylserine transport machinery that operates at ER-PM contact sites.Nature communications · 2026Article
- Identifying 2PHDO as a SOCE Inhibitor from Chinese Herbal Extracts.Current pharmaceutical biotechnology · 2026Article
- New insights into the stromal interaction molecule 2 function and its impact on the immunomodulation of tumor microenvironment.Cell & bioscience · 2024Review
- Redox Enzymes P4HB and PDIA3 Interact with STIM1 to Fine-Tune Its Calcium Sensitivity and Activation.International journal of molecular sciences · 2024Article
- Article
- An apical Phe-His pair defines the Orai1-coupling site and its occlusion within STIM1.Nature communications · 2023Article
- Engineering of NEMO as calcium indicators with large dynamics and high sensitivity.Nature methods · 2023Article
- Celastrol inhibits store operated calcium entry and suppresses psoriasis.Frontiers in pharmacology · 2023Article
- X-ray irradiation triggers immune response in human T-lymphocytes via store-operated Ca2+ entry and NFAT activation.The Journal of general physiology · 2022Article
- Identification of a STIM1 Splicing Variant that Promotes Glioblastoma Growth.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- STIM and Orai Mediated Regulation of Calcium Signaling in Age-Related Diseases.Frontiers in aging · 2022Review
- Omnitemporal choreographies of all five STIM/Orai and IPCell reports · 2021Article
- Engineering of a bona fide light-operated calcium channel.Nature communications · 2021Article
- Store-Operated Calcium Entry in the Cardiovascular System.Advances in experimental medicine and biology · 2021Review
- STIM Proteins: An Ever-Expanding Family.International journal of molecular sciences · 2020Review
- Synergistic stabilization by nitrosoglutathione-induced thiol modifications in the stromal interaction molecule-2 luminal domain suppresses basal and store operated calcium entry.Scientific reports · 2020Article
- Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling.Nature communications · 2020Article
- The Endoplasmic Reticulum-Plasma Membrane Junction: A Hub for Agonist Regulation of CaCold Spring Harbor perspectives in biology · 2020Review
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 2 countries.
Funding
Abstract
The endoplasmic reticulum (ER) Ca2+ sensors stromal interaction molecule 1 (STIM1) and STIM2, which connect ER Ca2+ depletion with extracellular Ca2+ influx, are crucial for the maintenance of Ca2+ homeostasis in mammalian cells. Despite the recent progress in unraveling the role of STIM2 in Ca2+ signaling, the mechanistic underpinnings of its activation remain underexplored. We use an engineering approach to direct ER-resident STIMs to the plasma membrane (PM) while maintaining their correct membrane topology, as well as Förster resonance energy transfer (FRET) sensors that enabled in cellulo real-time monitoring of STIM activities. This allowed us to determine the calcium affinities of STIM1 and STIM2 both in cellulo and in situ, explaining the current discrepancies in the literature. We also identified the key structural determinants, especially the corresponding G residue in STIM1, which define the distinct activation dynamics of STIM2. The chimeric E470G mutation could switch STIM2 from a slow and weak Orai channel activator into a fast and potent one like STIM1 and vice versa. The systemic dissection of STIM2 activation by protein engineering sets the stage for the elucidation of the regulation and function of STIM2-mediated signaling in mammals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.