Evidence map›Paper›PMID 30444582›Full record

ArticleCurrent protocols in microbiology2019

Encephalitozoon: Tissue Culture, Cryopreservation, and Murine Infection.

Bing Han, Magali Moretto, Louis M Weiss

Open access · greenAbstract read
In one paragraph

Article in Current protocols in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Crotoxin Upregulating NLRP-3 Inflammasome and IL-18 and Activating CD4Animals : an open access journal from MDPI · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Chemical disinfection ofFrontiers in microbiology · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. Von Willebrand Factor Facilitates Intravascular Dissemination of MicrosporidiaFrontiers in cellular and infection microbiology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Bing HanDepartment of Pathology, Division of Tropical Medicine and Parasitology, Albert Einstein College of Medicine, Bronx, New York.
Magali MorettoDepartment of Microbiology, Immunology, and Tropical Medicine, George Washington University, Washington, D.C.
Louis M WeissDepartment of Pathology, Division of Tropical Medicine and Parasitology, Albert Einstein College of Medicine, Bronx, New York.
Albert Einstein College of Medicine · US

Funding

Microsporidia: invasion apparatusR01AI124753 · NIAID · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI WEISS, LOUIS M. · 2016 to 2025
$4.2M
Development of Novel MetAP2 inhibitors as potential therapeutics for MicrosporidiosisR01AI132614 · NIAID · LONG ISLAND UNIVERSITY BROOKLYN CAMPUS · PI DAS, BHASKAR CHANDRA, WEISS, LOUIS M. · 2018 to 2022
$2.5M
NIAID NIH HHS R01 AI124753NIAID NIH HHS R01 AI132614
6 · The paper itself

Abstract

Microsporidia are eukaryotic unicellular parasites that have been studied for more than 150 years. They are found throughout the world and are capable of infecting various invertebrate and vertebrate hosts. They can cause disease in both immune-compromised and immune-competent humans. In immune-compromised individuals, infections can be severe and often fatal. Microsporidia possess a unique, highly specialized invasion mechanism that involves a structure known as the polar tube as well as the spore wall. During spore germination, the polar tube rapidly discharges from the spore and deliver the sporoplasm into the host cell. Spores are the only stage of microsporidia that can survive outside of host cells. Since the first attempt to culture microsporidia in vitro in 1930s, their cultivation has served a critical role in the study and diagnosis of these parasites. In this chapter, we include methods on the cultivation, isolation, and cryopreservation of Encephalitozoon cuniculi, which can infect humans and provides a useful model for other microsporidia. These methods can also be utilized for the culture of Encephalitozoon hellem or Encephalitozoon intestinalis. © 2018 by John Wiley & Sons, Inc.

Indexed as

Disease Models, AnimalAnimalsContainment of BiohazardsCryopreservationEncephalitozoonEncephalitozoonosisHumansMicePathologySpores, FungalTissue Culture Techniquesanimal modelcell passage and maintenancecryopreservationEncephalitozoonmicrosporidiaPCRRK13 cells

Identifiers

PMID30444582
PMCPMC6340769
OpenAlexW2900463751

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.