Evidence map›Paper›PMID 30444308›Full record

ArticleCurrent protocols in microbiology2019

Infection and Propagation of Astrovirus VA1 in Cell Culture.

Andrew B Janowski, David Wang

Open access · greenAbstract read
In one paragraph

Article in Current protocols in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 30 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Antiviral activity of ribavirin and favipiravir against human astroviruses.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020
    Article
  8. DifferentialmBio · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Andrew B JanowskiWashington University School of Medicine, St. Louis, Missouri.
David WangWashington University School of Medicine, St. Louis, Missouri.
Washington University in St. Louis · US

Funding

CHARACTERIZATION OF ASTROVIRUS VA1, A NOVEL NEUROTROPIC VIRUSK08AI132745 · NIAID · WASHINGTON UNIVERSITY · PI JANOWSKI, ANDREW BOK SENG · 2018 to 2022
$809k
PROPAGATION OF THE EMERGING ENCEPHALITIC VIRUS ASTROVIRUS VA1R21NS101371 · NINDS · WASHINGTON UNIVERSITY · PI WANG, DAVID · 2017 to 2018
$419k
NIAID NIH HHS K08 AI132745NINDS NIH HHS R21 NS101371
6 · The paper itself

Abstract

Astrovirus VA1/HMO-C (VA1) is the representative genotype of mamastrovirus 9, a species of the single-stranded, positive-sense RNA viral family, Astroviridae. Astroviruses have been traditionally considered pathogens of the gastrointestinal tract but they have been recently associated with neurological diseases in humans, cattle, mink, sheep, and pigs. VA1 is the astrovirus genotype most commonly identified from human cases of meningoencephalitis and has been recently propagated in cell culture. VA1 can now be used as a model system to study pathogenesis of the neurological diseases associated with astrovirus infection. In this article, we describe two fundamental assays to quantify replication and propagation of VA1, a quantitative reverse transcription-PCR (qRT-PCR) to measure viral RNA and a 50% tissue culture infectious dose (TCID

Indexed as

AstroviridaeAstroviridae InfectionsCaco-2 CellsCell Culture TechniquesHumansReal-Time Polymerase Chain ReactionRNA, ViralVirus CultivationRNA, ViralAstrovirusesastrovirus VA1cell culturereplicationtitration

Identifiers

PMID30444308
PMCPMC6340763
OpenAlexW2900813190

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.