Evidence map›Paper›PMID 30443837›Full record

ArticleMarine biotechnology (New York, N.Y.)2019

Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica.

P R Gabbai-Armelin, H W Kido, M A Cruz, J P S Prado, I R Avanzi, M R Custódio, A C M Renno, R N Granito

Abstract read
PubMed Publisher
In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 28 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

P R Gabbai-ArmelinLaboratory of Biomaterials and Tissue Engineering, Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim, 136, Santos, SP, 11015-020, Brazil. paulogabbai@gmail.com.ORCID http://orcid.org/0000-0003-4802-0062
H W KidoLaboratory of Biomaterials and Tissue Engineering, Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim, 136, Santos, SP, 11015-020, Brazil.
M A CruzLaboratory of Biomaterials and Tissue Engineering, Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim, 136, Santos, SP, 11015-020, Brazil.
J P S PradoLaboratory of Biomaterials and Tissue Engineering, Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim, 136, Santos, SP, 11015-020, Brazil.
I R AvanziLaboratory of Biomaterials and Tissue Engineering, Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim, 136, Santos, SP, 11015-020, Brazil.
M R CustódioLaboratory of Marine Invertebrates Cell Biology, Institute of Biosciences, University of São Paulo (USP), Rua do Matão, 101, São Paulo, SP, 05508-900, Brazil.
A C M RennoLaboratory of Biomaterials and Tissue Engineering, Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim, 136, Santos, SP, 11015-020, Brazil.
R N GranitoLaboratory of Biomaterials and Tissue Engineering, Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim, 136, Santos, SP, 11015-020, Brazil.
Universidade Federal de São Paulo · BRUniversidade de São Paulo · BR

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2014/23471-1Fundação de Amparo à Pesquisa do Estado de São Paulo 2015/20704-8
6 · The paper itself

Abstract

Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Thus, the objectives of this study were to study the physicochemical and morphological properties of biosilica (BS) from sponges by using scanning electron microscopy, Fourier-transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mineralization, and pH. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) were performed to investigate its cytotoxicity and proliferation in presence of BS. Bioglass (BG) was used as gold standard material for comparison purposes. Sponge BS was obtained, and this fact was proven by SEM, FTIR, and XRD analysis. Calcium assay showed a progressive release of this ion from day 7 and a more balanced pH for BS was maintained compared to BG. Cytotoxicity assay indicated that BS had a positive influence on MC3T3-E1 cells viability and qRT-PCR showed that this material stimulated Runx2 and BMP4 gene expressions. Taken together, the results indicate a potential use of sponge biosilica for tissue engineering applications.

Indexed as

AnimalsBiocompatible MaterialsBiomarkersBone and BonesBone Morphogenetic Protein 4Bone SubstitutesCell LineCell ProliferationCell SurvivalCore Binding Factor Alpha 1 SubunitFractures, BoneGene ExpressionHumansMiceOsteoblastsPoriferaBiocompatible MaterialsBiomarkersBmp4 protein, mouseBone Morphogenetic Protein 4Bone SubstitutesCore Binding Factor Alpha 1 SubunitRunx2 protein, mouseSilicon DioxideBioglassBiosilicaBone repairCytotoxicityPorifera

Identifiers

PMID30443837
OpenAlexW2901200626

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.