ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2018
A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
- Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human.Scientific reports · 2023Article
- Comparative studies of X chromosomes in Cervidae family.Scientific reports · 2023Article
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- The Cytogenetic Map of the Nile Crocodile (International journal of molecular sciences · 2022Article
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- Repetitive Sequence Distribution onBiology · 2021Article
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- Karyotypic and molecular evidence supports the endemic Tibetan hamsters as a separate divergent lineage of Cricetinae.Scientific reports · 2021Article
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- Dynamic RNA Regulation in the Brain Underlies Physiological Plasticity in a Hibernating Mammal.Frontiers in physiology · 2020Article
- Comparative Chromosome Mapping of Musk Ox and the X Chromosome among Some Bovidae Species.Genes · 2019Article
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8 authors.
Funding
Abstract
Сonstitutive heterochromatin areas are revealed by differential staining as C-positive chromosomal regions. These C-positive bands may greatly vary by location, size, and nucleotide composition. CBG-banding is the most commonly used method to detect structural heterochromatin in animals. The difficulty in identification of individual chromosomes represents an unresolved problem of this method as the body of the chromosome is stained uniformly and does not have banding pattern beyond C-bands. Here, we present the method that we called CDAG for sequential heterochromatin staining after differential GTG-banding. The method uses G-banding followed by heat denaturation in the presence of formamide with consecutive fluorochrome staining. The new technique is valid for the concurrent revealing of heterochromatin position due to differential banding of chromosomes and heterochromatin composition (AT-/GC-rich) in animal karyotyping.
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