ArticlePediatric surgery international2019
Whole rat stomach decellularisation using a detergent-enzymatic protocol.
Article in Pediatric surgery international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- dECM bioinks for 3D bioprinted tumor models: Advances, challenges, and drug screening.iScience · 2026Review
- Application of tergitol in liver decellularization and bioink preparation based on the obtained extracellular matrix.Frontiers in bioengineering and biotechnology · 2025Article
- Stomach engineering: region-specific characterization of the decellularized porcine stomach.Pediatric surgery international · 2023Article
- Zinc Deficiency Exacerbates Bisphenol A-Induced Hepatic and Renal Damage: Delineation of Molecular Mechanisms.Biological trace element research · 2023Article
- Cardiac-derived extracellular matrix: A decellularization protocol for heart regeneration.PloS one · 2022Article
- 3D bioprinting of multi-layered segments of a vessel-like structure with ECM and novel derived bioink.Frontiers in bioengineering and biotechnology · 2022Article
- 3D Printing: Advancement in Biogenerative Engineering to Combat Shortage of Organs and Bioapplicable Materials.Regenerative engineering and translational medicine · 2022Review
- Matrix-Bound Nanovesicles: The Effects of Isolation Method upon Yield, Purity, and Function.Tissue engineering. Part C, Methods · 2020Article
- M. leprae interacts with the human epidermal keratinocytes, neonatal (HEKn) via the binding of laminin-5 with α-dystroglycan, integrin-β1, or -β4.PLoS neglected tropical diseases · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConditions leading to reduced gastric volume are difficult to manage and are associated to poor quality-of-life. Stomach augmentation using a tissue-engineered stomach is a potential solution to restore adequate physiology and food reservoir. Aim of this study was to evaluate the decellularisation of whole rat stomach using a detergent-enzymatic protocol.
methodsStomachs harvested from rats were decellularised through luminal and vascular cannulation using 24-h detergent-enzymatic treatment and completely characterized by appropriate staining, DNA and Extracellular matrix -component quantifications.
resultsThe detergent-enzymatic protocol allows a complete decellularisation of the gastric tissue, with a complete removal of the DNA with two cycles as confirmed by both quantifications and histological analysis. Extracellular matrix components, collagen, fibronectin, laminin and elastin, were optimally preserved by the treatment, while glycosaminoglycans were reduced.
conclusionGastric tissue can be efficiently decellularised. Scaffolds retained original structure and important components that could enhance integration with other tissues for in vivo transplant. The use of naturally derived material could be potentially considered for the treatment of both congenital and acquired conditions.
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