ArticleScientific reports2018
RNA sequencing-based analysis of the laying hen uterus revealed the novel genes and biological pathways involved in the eggshell biomineralization.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 92 citations in OpenAlex.
- A mugwort-based Chinese herbal mixture of five herbs improved oviduct health in laying hens under LPS challenge.Poultry science · 2026Article
- Deciphering the uterine transcriptional mechanisms underlying eggshell strength through multi-stage analysis in laying hens.Poultry science · 2026Article
- Multi-tissue transcriptomic profiling reveals the internal physiological landscape of laying hens in cage and cage-free systems.Poultry science · 2026Article
- Age-associated changes in the shell gland transcriptomics and eggshell quality of broiler breeder hens.BMC genomics · 2026Article
- Optimizing eggshell quality in extended laying periods: Insights and strategies.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Review
- Uterine fluid proteomic and metabolomic profiles associated with soft-shelled egg formation in laying hens.Frontiers in veterinary science · 2026Article
- Regulatory effects of hawthorn leaf flavonoids and stevioside on the uterine function and eggshell quality in laying hens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Integrated mRNA-seq and miRNA-seq analysis reveals miR-210a-5p regulates uterine aging in laying hens by targeting the RASL11B/Raf/MAPK pathway.Journal of animal science and biotechnology · 2025Article
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- Puerarin Enhances Eggshell Quality by Mitigating Uterine Senescence in Late-Phase Laying Breeder Hens.Antioxidants (Basel, Switzerland) · 2025Article
- Egg Quality and Nutritional Profile of Three Sicilian Autochthonous Chicken Breeds: Siciliana, Cornuta di Caltanissetta, and Valplatani.Foods (Basel, Switzerland) · 2025Article
- Adaptive Evolution ofMicroorganisms · 2025Article
- Transcriptome-based analysis reveals relationship between duck eggshell color and eggshell strength.BMC genomics · 2025Article
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- Cross-species regulatory network analysis identifies FOXO1 as a driver of ovarian follicular recruitment.Scientific reports · 2024Article
- Structural mechanism of proton conduction in otopetrin proton channel.Nature communications · 2024Article
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- Transcriptome profiling analysis of uterus during chicken laying periods.BMC genomics · 2023Article
- Uterine inflammation status modulates eggshell mineralization via calcium transport and matrix protein synthesis in laying hens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Eggshell is the outermost calcified covering of an egg that protects it from microbial invasion and physical damage, and is critical for egg quality. However, understanding of the genes/proteins and the biological pathways regulating the eggshell formation is still obscure. We hypothesized that the transcriptomic analysis of the chicken uteri using RNA-sequencing may reveal novel genes and biological pathways involved in the eggshell biomineralization. RNA-sequence analysis using uteri of laying hens at 15-20 h post-ovulation (layers, n = 3) and non-laying (non-layers, n = 3) hens was carried out. About 229 differentially expressed genes (DEGs) were up-regulated in the layers compared to the non-layers. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Ingenuity Pathway Analysis (IPA) revealed more than ten novel genes and biological pathways related to calcium transport and mineralization in the uterus. Based on the enriched pathways and molecular function analysis, 12 DEGs related to eggshell mineralization were further analyzed in the uteri of layers (3 h and 15-20 h post-ovulation), non-layers and molters using qPCR. Expressions of OC-116 (regulator of mineralization), OTOP2 (modulator of cellular calcium influx), CALCB (intracellular release of Ca-ions), STC2 (increases alkaline phosphatase activity), and ATP2C2 (cellular import of Ca-ions) were significantly higher in the uteri of laying hen at 15-20 h post-ovulation. This study identified the involvement of novel genes and their proposed biological pathways in the regulation of eggshell formation.
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