ArticleScientific reports2018
A Rabbit Corneal Endothelial Dysfunction Model Using Endothelial-Mesenchymal Transformed Cells.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 20 citations in OpenAlex.
- Outcomes of Ahmed Glaucoma Valve Placement in Client-Owned Rabbits With Refractory Glaucoma.Veterinary ophthalmology · 2026Article
- High-Purity Functional Corneal Endothelial Cells From Human Induced Pluripotent Stem Cells via a Novel Wash-Out Method.MedComm · 2026Article
- Porcine Corneal Models as Translational Platforms for Innovative Therapies: Current Insights and Future Directions.Journal of functional biomaterials · 2025Review
- Ultrastructural and Immunohistochemical Study on the Nephrotoxicity Following Intravitreal Administration of the Antifungal Agents Voriconazole and Micafungin in New Zealand White Rabbits.International journal of molecular sciences · 2025Article
- Use of femtosecond laser generated reactive oxygen species to manage inflammation and enhance wound healing after ocular trauma.Scientific reports · 2025Article
- Nanomedicine in Ophthalmology: From Bench to Bedside.Journal of clinical medicine · 2024Review
- Hepatocyte Growth Factor Modulates Corneal Endothelial Wound Healing In Vitro.International journal of molecular sciences · 2024Article
- Focus on seed cells: stem cells in 3D bioprinting of corneal grafts.Frontiers in bioengineering and biotechnology · 2024Review
- Non-viral gene therapy using RNA interference with PDGFR-α mediated epithelial-mesenchymal transformation for proliferative vitreoretinopathy.Materials today. Bio · 2023Article
- Six-month effective treatment of corneal graft rejection.Science advances · 2023Article
- Assessing Corneal Endothelial Damage Using Terahertz Time-Domain Spectroscopy and Support Vector Machines.Sensors (Basel, Switzerland) · 2022Article
- Corneal endothelial transplantation from bench to bedside: A review of animal models and their translational value for therapeutic development.Experimental eye research · 2022Review
- Fluctuations in Corneal Endothelial LAP2 Expression Levels Correlate with Passage Dependent Declines in Their Cell Proliferative Activity.International journal of molecular sciences · 2022Article
- Pluripotent stem cell-derived corneal endothelial cells as an alternative to donor corneal endothelium in keratoplasty.Stem cell reports · 2021Article
- Animal models of corneal endothelial dysfunction to facilitate development of novel therapies.Annals of translational medicine · 2021Review
- Transplantation of human induced pluripotent stem cell-derived neural crest cells for corneal endothelial regeneration.Stem cell research & therapy · 2021Article
- Experimental design of a culture approach for corneal endothelial cells of New Zealand white rabbit.Heliyon · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unlike humans, rabbit corneal endothelial wounds are known to spontaneously heal. The current study was aimed to develop a new rabbit bullous keratopathy model using corneal endothelial cells that were induced to undergo endothelial-mesenchymal transformation (EMT). EMT was induced in rabbit corneal endothelial cells (RCECs) by culturing with TGFβ and basic FGF Supplemented Medium. The corneal endothelia in recipient rabbits were mechanically scraped from the corneal endothelial surface inside an 8 mm mark. Then, a suspension of EMT-induced RCECs (EMT-RCECs) was injected into the anterior chamber. Eyes injected with freshly isolated RCECs (Fresh RCECs group) and eyes that were scraped without injection of cells (Scrape group) were used as controls. Immediately following operation, subepithelial and stromal edema was observed with increased central corneal thickness and corneal opacity in all groups. In the EMT-RCECs group, bullous keratopathy persisted for 42 days up to the end of the study. In the Fresh-RCECs and Scrape groups, corneal transparency and thickness recovered by 7 days after treatment and was maintained up to 42 days. The activated fibroblast marker, α-SMA, was observed spanning from corneal endothelium to corneal stroma in the EMT-RCECs group. Interestingly, α-SMA was upregulated in the Scrape-group as well. In all groups, there was no damage to other intraocular structures, and intraocular pressure was normal throughout the observation period. Transplanting a fresh donor cornea effectively treated corneal edema due to bullous keratopathy. This model is a promising tool for pre-clinical trials in the development of new therapies against corneal endothelial dysfunction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.