Evidence map›Paper›PMID 30442909›Full record

ReviewNature reviews. Microbiology2019

Mycobiome diversity: high-throughput sequencing and identification of fungi.

R Henrik Nilsson, Sten Anslan, Mohammad Bahram, Christian Wurzbacher, Petr Baldrian, Leho Tedersoo

Abstract readReview
PubMed Publisher
In one paragraph

Review in Nature reviews. Microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 377 papers.

0numbers the graph read from it
0cells of the map it votes in
377citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

377 citing papers in PubMed.

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  13. Legacy Effects ofJournal of fungi (Basel, Switzerland) · 2026
    Article
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  16. Review
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  20. Hidden Fungal DNA Structures May Shape Sequencing Outcomes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026
    Article

317 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

R Henrik NilssonGothenburg Global Biodiversity Centre, Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden. henrik.nilsson@bioenv.gu.se.ORCID http://orcid.org/0000-0002-8052-0107
Sten AnslanZoological Institute, Braunschweig University of Technology, Braunschweig, Germany.
Mohammad BahramDepartment of Ecology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Christian WurzbacherChair of Urban Water Systems Engineering, Technical University of Munich, Garching, Germany.
Petr BaldrianLaboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences, Praha, Czech Republic.ORCID http://orcid.org/0000-0002-8983-2721
Leho TedersooNatural History Museum of Tartu University, Tartu, Estonia.ORCID http://orcid.org/0000-0002-1635-1249

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fungi are major ecological players in both terrestrial and aquatic environments by cycling organic matter and channelling nutrients across trophic levels. High-throughput sequencing (HTS) studies of fungal communities are redrawing the map of the fungal kingdom by hinting at its enormous - and largely uncharted - taxonomic and functional diversity. However, HTS approaches come with a range of pitfalls and potential biases, cautioning against unwary application and interpretation of HTS technologies and results. In this Review, we provide an overview and practical recommendations for aspects of HTS studies ranging from sampling and laboratory practices to data processing and analysis. We also discuss upcoming trends and techniques in the field and summarize recent and noteworthy results from HTS studies targeting fungal communities and guilds. Our Review highlights the need for reproducibility and public data availability in the study of fungal communities. If the associated challenges and conceptual barriers are overcome, HTS offers immense possibilities in mycology and elsewhere.

Indexed as

BiodiversityHigh-Throughput Nucleotide SequencingMycobiomeDNA Barcoding, TaxonomicFungiReproducibility of Results

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.