ArticleScientific reports2018
Pseudomonas aeruginosa mutants defective in glucose uptake have pleiotropic phenotype and altered virulence in non-mammal infection models.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Phytochemicals as Invaluable Sources of Potent Antimicrobial Agents to Combat Antibiotic Resistance.Life (Basel, Switzerland) · 2023Review
- Commensal colonization reducesFrontiers in cellular and infection microbiology · 2023Article
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- Periodically Disturbing the Spatial Structure of Biofilms Can Affect the Production of an Essential Virulence Factor inmSystems · 2021Article
- Searching for Biological Function of the Mysterious PA2504 Protein fromInternational journal of molecular sciences · 2021Article
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- Dynamic Adaptive Response ofAntibiotics (Basel, Switzerland) · 2021Article
- DifferentMicroorganisms · 2021Article
- Transcriptomic Analysis ofFoods (Basel, Switzerland) · 2021Article
- Glucose-Binding of Periplasmic Protein GltB Activates GtrS-GltR Two-Component System inMicroorganisms · 2021Article
- Sanguinarine Inhibits the 2-Ketogluconate Pathway of Glucose Utilization inFrontiers in microbiology · 2021Article
- Overexpression ofMicroorganisms · 2020Article
- Activity and Impact on Resistance Development of Two Antivirulence Fluoropyrimidine Drugs inFrontiers in cellular and infection microbiology · 2019Article
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Authors and funding
10 authors.
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Abstract
Pseudomonas spp. are endowed with a complex pathway for glucose uptake that relies on multiple transporters. In this work we report the construction and characterization of Pseudomonas aeruginosa single and multiple mutants with unmarked deletions of genes encoding outer membrane (OM) and inner membrane (IM) proteins involved in glucose uptake. We found that a triple ΔgltKGF ΔgntP ΔkguT mutant lacking all known IM transporters (named GUN for Glucose Uptake Null) is unable to grow on glucose as unique carbon source. More than 500 genes controlling both metabolic functions and virulence traits show differential expression in GUN relative to the parental strain. Consistent with transcriptomic data, the GUN mutant displays a pleiotropic phenotype. Notably, the genome-wide transcriptional profile and most phenotypic traits differ between the GUN mutant and the wild type strain irrespective of the presence of glucose, suggesting that the investigated genes may have additional roles besides glucose transport. Finally, mutants carrying single or multiple deletions in the glucose uptake genes showed attenuated virulence relative to the wild type strain in Galleria mellonella, but not in Caenorhabditis elegans infection model, supporting the notion that metabolic functions may deeply impact P. aeruginosa adaptation to specific environments found inside the host.
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