ArticleACS synthetic biology2018
Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution.
Article in ACS synthetic biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Optogenetic manipulation of estrogen receptor signaling to improve estrogen deficiency.iScience · 2026Article
- Protein design accelerates the development and application of optogenetic tools.Computational and structural biotechnology journal · 2025Review
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Synthetic peptides for the precise transportation of proteins of interests to selectable subcellular areas.Frontiers in bioengineering and biotechnology · 2023Article
- LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics.Biophysics and physicobiology · 2023Article
- Nanoscale programming of cellular and physiological phenotypes: inorganic meets organic programming.NPJ systems biology and applications · 2021Review
- Optogenetic Tools for Manipulating Protein Subcellular Localization and Intracellular Signaling at Organelle Contact Sites.Current protocols · 2021Article
- Photoreaction Mechanisms of Flavoprotein Photoreceptors and Their Applications.Advances in experimental medicine and biology · 2021Review
- Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells.eLife · 2020Article
- SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique.PloS one · 2020Article
- Engineering Optogenetic Protein Analogs.Methods in molecular biology (Clifton, N.J.) · 2020Article
- FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics.Nature methods · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Optogenetic techniques use light-responsive proteins to study dynamic processes in living cells and organisms. These techniques typically rely on repurposed naturally occurring light-sensitive proteins to control subcellular localization and activity. We previously engineered two optogenetic systems, the light activated nuclear shuttle (LANS) and the light-inducible nuclear exporter (LINX), by embedding nuclear import or export sequence motifs into the C-terminal helix of the light-responsive LOV2 domain of Avena sativa phototropin 1, thus enabling light-dependent trafficking of a target protein into and out of the nucleus. While LANS and LINX are effective tools, we posited that mutations within the LOV2 hinge-loop, which connects the core PAS domain and the C-terminal helix, would further improve the functionality of these switches. Here, we identify hinge-loop mutations that favorably shift the dynamic range (the ratio of the on- to off-target subcellular accumulation) of the LANS and LINX photoswitches. We demonstrate the utility of these new optogenetic tools to control gene transcription and epigenetic modifications, thereby expanding the optogenetic "tool kit" for the research community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.