Evidence map›Paper›PMID 30441776›Full record

ArticleSensors (Basel, Switzerland)2018

Sandwich Electrochemical Immunosensor for Early Detection of Tuberculosis Based on Graphene/Polyaniline-Modified Screen-Printed Gold Electrode.

Umi Zulaikha Mohd Azmi, Nor Azah Yusof, Norzila Kusnin, Jaafar Abdullah, Siti Suraiya, Poh Shing Ong, Nurul Hanun Ahmad Raston, Siti Fatimah Abd Rahman, Mohamad Faris Mohamad Fathil

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  11. Aptasensor for the Detection ofNanomaterials (Basel, Switzerland) · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Umi Zulaikha Mohd AzmiInstitute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. umizulaikha.ika@gmail.com.
Nor Azah YusofInstitute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. azahy@upm.edu.my.
Norzila KusninInstitute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. norzilakusnin87@gmail.com.
Jaafar AbdullahInstitute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. jafar@upm.edu.my.
Siti SuraiyaSchool of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia. ssuraiya@usm.my.
Poh Shing OngNanoMalaysia Berhad, a CLG under the Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Kuala Lumpur 50450, Malaysia. pohshing.ong@nanomalaysia.com.my.
Nurul Hanun Ahmad RastonSchool of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi 43600, Selangor, Malaysia. nurulhanun@ukm.edu.my.
Siti Fatimah Abd RahmanInstitute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. siti_fatimahar@upm.edu.my.
Mohamad Faris Mohamad FathilInstitute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Perlis, Malaysia. mohamadfaris@unimap.edu.my.

Funding

Nano Malaysia Berhad 03-01-04-SF2160
6 · The paper itself

Abstract

A rapid and sensitive sandwich electrochemical immunosensor was developed based on the fabrication of the graphene/polyaniline (GP/PANI) nanocomposite onto screen-printed gold electrode (SPGE) for detection of tuberculosis biomarker 10-kDa culture filtrate protein (CFP10). The prepared GP/PANI nanocomposite was characterized using Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM). The chemical bonding and morphology of GP/PANI-modified SPGE were studied by Raman spectroscopy and FESEM coupled with energy dispersive X-ray spectroscopy, respectively. From both studies, it clearly showed that GP/PANI was successfully coated onto SPGE through drop cast technique. Cyclic voltammetry was used to study the electrochemical properties of the modified electrode. The effective surface area for GP/PANI-modified SPGE was enhanced about five times compared with bare SPGE. Differential pulse voltammetry was used to detect the CFP10 antigen. The GP/PANI-modified SPGE that was fortified with sandwich type immunosensor exhibited a wide linear range (20⁻100 ng/mL) with a low detection limit of 15 ng/mL. This proposed electrochemical immunosensor is sensitive, low sample volume, rapid and disposable, which is suitable for tuberculosis detection in real samples.

Indexed as

Biosensing TechniquesAniline CompoundsEarly DiagnosisElectrochemical TechniquesGraphiteHumansLimit of DetectionMetal NanoparticlesMicroscopy, Electron, ScanningMycobacterium tuberculosisPeptide FragmentsSpectroscopy, Fourier Transform InfraredSpectrum Analysis, RamanTuberculosisAniline CompoundsCFP-10 protein (71-85), Mycobacterium tuberculosisGraphitePeptide Fragmentspolyanilineelectrochemical sensorgraphenepolyanilinescreen-printed gold electrode (SPGE)tuberculosis

Identifiers

PMID30441776
PMCPMC6263639

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.