Evidence map›Paper›PMID 30440001›Full record

ArticlePloS one2018

Yeast XRS2 and human NBN gene: Experimental evidence for homology using codon optimized cDNA.

Ilja Demuth, Simon K Krebs, Véronique Dutrannoy, Christian Linke, Sylvia Krobitsch, Raymonda Varon, Christine Lang, Andreas Raab, Karl Sperling, Martin Digweed

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Ilja DemuthCharité -Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Lipid Clinic at the Interdisciplinary Metabolism Center, Berlin, Germany.ORCID 0000-0002-4340-2523
Simon K KrebsInstitute of Biotechnology, Technical University Berlin, Berlin, Germany.
Véronique DutrannoyCharité -Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Medical and Human Genetics, Berlin, Germany.
Christian LinkeOtto Warburg Laboratory, Max Planck Institute for Molecular Genetics, Berlin, Germany.
Sylvia KrobitschOtto Warburg Laboratory, Max Planck Institute for Molecular Genetics, Berlin, Germany.
Raymonda VaronCharité -Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Medical and Human Genetics, Berlin, Germany.
Christine LangORGANOBALANCE GmbH, Berlin, Germany.
Andreas RaabORGANOBALANCE GmbH, Berlin, Germany.
Karl SperlingCharité -Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Medical and Human Genetics, Berlin, Germany.ORCID 0000-0003-2305-4370
Martin DigweedCharité -Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Medical and Human Genetics, Berlin, Germany.
Humboldt-Universität zu Berlin · DEMax Planck Institute for Molecular Genetics · DETechnische Universität Berlin · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genes, XRS2 in Saccharomyces cerevisiae and NBN in mammals, have little sequence identity at the amino acid level. Nevertheless, they are both found together with MRE11 and RAD50 in a highly conserved protein complex which functions in the repair of DNA double-strand breaks. Here, we have examined the evolutionary and functional relationship of these two genes by cross-complementation experiments. These experiments necessitated sequence correction for specific codon usage before they could be successfully conducted. We present evidence that despite extreme sequence divergence nibrin can, at least partially, replace Xrs2 in the cellular DNA damage response, and Xrs2 is able to promote nuclear localization of MRE11 in NBS cells. We discuss that the extreme sequence divergence reflects a unique adaptive pressure during evolution related to the specific eukaryotic role for both Xrs2 and nibrin in the subcellular localisation of the DNA repair complex. This, we suggest, is of particular relevance when cells are infected by viruses. The conflict hypothesis of co-evolution of DNA repair genes and DNA viruses may thus explain the very low sequence identity of these two homologous genes.

Indexed as

Cell Cycle ProteinsCodonDNA DamageGenetic Complementation TestNuclear ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsCell Line, TransformedHumansCell Cycle ProteinsCodonNBN protein, humanNuclear ProteinsSaccharomyces cerevisiae ProteinsXRS2 protein, S cerevisiae

Identifiers

PMID30440001
PMCPMC6237358
OpenAlexW2901749801

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.