Evidence map›Paper›PMID 30431194›Full record

ArticleNMR in biomedicine2019

Three-dimensional NMR microscopy of zebrafish specimens.

Timothy L Kline, Caroline R Sussman, Maria V Irazabal, Prasanna K Mishra, Elisabeth A Pearson, Vicente E Torres, Slobodan I Macura

Abstract read
In one paragraph

Article in NMR in biomedicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Brain Structures in a Human Embryo Imaged with MR Microscopy.Magnetic resonance in medical sciences : MRMS : an official journal of Japan Society of Magnetic Resonance in Medicine · 2025
    Article
  2. Cerebrovascular development: mechanisms and experimental approaches.Cellular and molecular life sciences : CMLS · 2021
    Review
  3. Article
  4. Tissue Transparency In Vivo.Molecules (Basel, Switzerland) · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Timothy L KlineDepartment of Radiology, Mayo Clinic, Rochester, MN, USA.ORCID 0000-0002-7917-9853
Caroline R SussmanDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, USA.ORCID 0000-0003-3965-256X
Maria V IrazabalDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, USA.ORCID 0000-0002-9904-7778
Prasanna K MishraBiochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.
Elisabeth A PearsonDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, USA.
Vicente E TorresDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, USA.ORCID 0000-0003-2008-1576
Slobodan I MacuraBiochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.ORCID 0000-0002-6054-5163

Funding

Pilot and FeasibilityP30DK090728 · NIDDK · MAYO CLINIC ROCHESTER · PI ERICKSON, BRADLEY J. · 2010 to 2019
$11.8M
RENIN ANGIOTENSIN SYSTEM IN THE PATHOGENESIS OF ADPKDR01DK044863 · NIDDK · MAYO CLINIC ROCHESTER · PI TORRES, VICENTE E · 1991 to 2019
$4.5M
Mayo Clinic Summer Undergraduate Research in Nephrology & UrologyR25DK101405 · NIDDK · MAYO CLINIC ROCHESTER · PI MICHAEL F. ROMERO · 2014 to 2026
$1.6M
Advanced MR Imaging and Image Analytics as a Precision Medicine Tool to Manage ADPKDK01DK110136 · NIDDK · MAYO CLINIC ROCHESTER · PI KLINE, TIMOTHY LEE · 2017 to 2021
$732k
Mayo Clinic Robert M. and Billie Kelley Pirnie Translational PKD CenterNIDD K01DK110136NIDD P30DK090728NIDDK NIH HHS K01 DK110136NIDDK NIH HHS P30 DK090728NIDDK NIH HHS R01 DK044863NIDDK NIH HHS R25 DK101405
6 · The paper itself

Abstract

While zebrafish embryos in the first five days after fertilization are clear and amenable to optical analysis, older juveniles and adults are not, due to pigmentation development and tissue growth. Thus other imaging methods are needed to image adult specimens. NMR is a versatile tool for studies of biological systems and has been successfully used for in vivo zebrafish microscopy. In this work we use NMR microscopy (MRM) for assessment of zebrafish specimens, which includes imaging of formalin fixed (FF), formalin fixed and paraffin embedded (FFPE), fresh (unfixed), and FF gadolinium doped specimens. To delineate the size and shape of various organs we concentrated on 3D MRM. We have shown that at 7 T a 3D NMR image can be obtained with isotropic resolution of 50 μm/pxl within 10 min and 25 μm/pxl within 4 h. Also, we have analyzed sources of contrast and have found that in FF specimens the best contrast is obtained by T

Indexed as

Imaging, Three-DimensionalMagnetic Resonance SpectroscopyAnimalsGadoliniumMicroscopyParaffin EmbeddingTissue FixationZebrafishGadoliniumFFFFPEformalin fixedmagnetic resonance microscopyMRINMRparaffin embeddedzebrafish

Identifiers

PMID30431194
PMCPMC6537090

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.