ArticlePlant reproduction2019
Adapting INTACT to analyse cell-type-specific transcriptomes and nucleocytoplasmic mRNA dynamics in the Arabidopsis embryo.
Article in Plant reproduction, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Transcription Profiling of Potato Leaves in Response to Heat Stress at Single-Cell Resolution.Plant biotechnology journal · 2026Article
- Detection and Automated Quantification of Nucleocytoplasmic RNA Fractions in Arabidopsis Using smFISH.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Current status and trends in forest genomics.Forestry research · 2022Review
- Endosperm-Embryo Communications: Recent Advances and Perspectives.Plants (Basel, Switzerland) · 2021Review
- Tissue-specific transcriptome profiling of the Arabidopsis inflorescence stem reveals local cellular signatures.The Plant cell · 2021Article
- Review
- TheQuantitative plant biology · 2021Review
- Genetic activity during early plant embryogenesis.The Biochemical journal · 2020Review
- Research Tools for the Functional Genomics of Plant miRNAs During Zygotic and Somatic Embryogenesis.International journal of molecular sciences · 2020Review
- Laser-Assisted Microdissection of Plant Embryos for Transcriptional Profiling.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Examining the contribution of histone modification to sex differences in learning and memory.Learning & memory (Cold Spring Harbor, N.Y.) · 2019Review
- Special issue: cellular omics methods in plant reproduction research.Plant reproduction · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the early embryo of vascular plants, the different cell types and stem cells of the seedling are specified as the embryo develops from a zygote towards maturity. How the key steps in cell and tissue specification are instructed by genome-wide transcriptional activity is poorly understood. Progress in defining transcriptional regulation at the genome-wide level in plant embryos has been hampered by difficulties associated with capturing cell-type-specific transcriptomes in this small and inaccessible structure. We recently adapted a two-component genetic nucleus labelling system called INTACT to isolate nuclei from distinct cell types at different stages of Arabidopsis thaliana embryogenesis. We have used these to generate a transcriptomic atlas of embryo development following microarray-based expression profiling. Here, we present a general description of the adapted INTACT procedure, including the two-component labelling system, seed isolation, nuclei preparation and purification, as well as transcriptomic profiling. We also compare nuclear and cellular transcriptomes from the early Arabidopsis embryo to assess nucleocytoplasmic differences and discuss how these differences can be used to infer regulation of gene activity.
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Registered trials
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