ArticleScientific reports2018
Terminal restriction fragment length polymorphism is an "old school" reliable technique for swift microbial community screening in anaerobic digestion.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence of unculturable bacteria in the periapical abscess: A systematic review and meta-analysis.PloS one · 2021Pooled it
- Fe(III) bioreduction kinetics in anaerobic batch and continuous stirred tank reactors with acidophilic bacteria relevant for bioleaching of limonitic laterites.Frontiers in microbiology · 2024Article
- Phosphate fertilization affects rhizosphere microbiome of maize and sorghum genotypes.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Engineering microbial technologies for environmental sustainability: choices to make.Microbial biotechnology · 2022Article
- Indicative Marker Microbiome Structures Deduced from the Taxonomic Inventory of 67 Full-Scale Anaerobic Digesters of 49 Agricultural Biogas Plants.Microorganisms · 2021Article
- Redox Potential Heterogeneity in Fixed-Bed Electrodes Leads to Microbial Stratification and Inhomogeneous Performance.ChemSusChem · 2021Article
- Effects of combined tannic acid/fluoride on sulfur transformations and methanogenic pathways in swine manure.PloS one · 2021Article
- Microbiome Diversity and Community-Level Change Points within Manure-based small Biogas Plants.Microorganisms · 2020Article
- Stochasticity in microbiology: managing unpredictability to reach the Sustainable Development Goals.Microbial biotechnology · 2020Article
- The next frontier of the anaerobic digestion microbiome: From ecology to process control.Environmental science and ecotechnology · 2020Review
- Effect of a Profound Feedstock Change on the Structure and Performance of Biogas Microbiomes.Microorganisms · 2020Article
- High-Throughput Sequencing and Unsupervised Analysis of Formyltetrahydrofolate Synthetase (FTHFS) Gene Amplicons to Estimate Acetogenic Community Structure.Frontiers in microbiology · 2020Article
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4 authors.
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Abstract
The microbial community in anaerobic digestion has been analysed through microbial fingerprinting techniques, such as terminal restriction fragment length polymorphism (TRFLP), for decades. In the last decade, high-throughput 16S rRNA gene amplicon sequencing has replaced these techniques, but the time-consuming and complex nature of high-throughput techniques is a potential bottleneck for full-scale anaerobic digestion application, when monitoring community dynamics. Here, the bacterial and archaeal TRFLP profiles were compared with 16S rRNA gene amplicon profiles (Illumina platform) of 25 full-scale anaerobic digestion plants. The α-diversity analysis revealed a higher richness based on Illumina data, compared with the TRFLP data. This coincided with a clear difference in community organisation, Pareto distribution, and co-occurrence network statistics, i.e., betweenness centrality and normalised degree. The β-diversity analysis showed a similar clustering profile for the Illumina, bacterial TRFLP and archaeal TRFLP data, based on different distance measures and independent of phylogenetic identification, with pH and temperature as the two key operational parameters determining microbial community composition. The combined knowledge of temporal dynamics and projected clustering in the β-diversity profile, based on the TRFLP data, distinctly showed that TRFLP is a reliable technique for swift microbial community dynamics screening in full-scale anaerobic digestion plants.
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