ArticlePharmaceutics2018
The Combined Effects of Co-Culture and Substrate Mechanics on 3D Tumor Spheroid Formation within Microgels Prepared via Flow-Focusing Microfluidic Fabrication.
Article in Pharmaceutics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Polydopamine Interfacial Coating for Stable Tumor-on-a-Chip Models: Application for Pancreatic Ductal Adenocarcinoma.Biomacromolecules · 2024Article
- Uniform sized cancer spheroids production using hydrogel-based droplet microfluidics: a review.Biomedical microdevices · 2024Review
- Microfluidics and Organoids, the Power Couple of Developmental Biology and Oncology Studies.International journal of molecular sciences · 2023Review
- Spheroid Engineering in Microfluidic Devices.ACS omega · 2023Review
- Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering.Chemical reviews · 2022Review
- Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter.Advanced materials technologies · 2022Article
- Easy-to-Operate Co-Flow Step Emulsification Device for High-Throughput Three-Dimensional Cell Culture.Biosensors · 2022Article
- Microfluidic Applications in Drug Development: Fabrication of Drug Carriers and Drug Toxicity Screening.Micromachines · 2022Review
- Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models.Research (Washington, D.C.) · 2022Article
- Droplet microfluidic devices for organized stem cell differentiation into germ cells: capabilities and challenges.Biophysical reviews · 2021Review
- Tissue engineered platforms for studying primary and metastatic neoplasm behavior in bone.Journal of biomechanics · 2021Review
- The utility of biomedical scaffolds laden with spheroids in various tissue engineering applications.Theranostics · 2021Review
- Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment.International journal of bioprinting · 2021Review
- The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review.Micromachines · 2020Review
- Microfluidic technologies to engineer mesenchymal stem cell aggregates-applications and benefits.Biophysical reviews · 2020Review
- Engineered microscale hydrogels for drug delivery, cell therapy, and sequencing.Biomedical microdevices · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Tumor spheroids are considered a valuable three dimensional (3D) tissue model to study various aspects of tumor physiology for biomedical applications such as tissue engineering and drug screening as well as basic scientific endeavors, as several cell types can efficiently form spheroids by themselves in both suspension and adherent cell cultures. However, it is more desirable to utilize a 3D scaffold with tunable properties to create more physiologically relevant tumor spheroids as well as optimize their formation. In this study, bioactive spherical microgels supporting 3D cell culture are fabricated by a flow-focusing microfluidic device. Uniform-sized aqueous droplets of gel precursor solution dispersed with cells generated by the microfluidic device are photocrosslinked to fabricate cell-laden microgels. Their mechanical properties are controlled by the concentration of gel-forming polymer. Using breast adenocarcinoma cells, MCF-7, the effect of mechanical properties of microgels on their proliferation and the eventual spheroid formation was explored. Furthermore, the tumor cells are co-cultured with macrophages of fibroblasts, which are known to play a prominent role in tumor physiology, within the microgels to explore their role in spheroid formation. Taken together, the results from this study provide the design strategy for creating tumor spheroids utilizing mechanically-tunable microgels as 3D cell culture platform.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.