Evidence map›Paper›PMID 30427751›Full record

ArticleMolecular biology of the cell2019

The J-domain cochaperone Rsp1 interacts with Mto1 to organize noncentrosomal microtubule assembly.

Juan Shen, Tianpeng Li, Xiaojia Niu, Wenyue Liu, Shengnan Zheng, Jing Wang, Fengsong Wang, Xinwang Cao, Xuebiao Yao, Fan Zheng and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Juan ShenDivision of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Tianpeng LiDivision of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Xiaojia NiuDivision of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Wenyue LiuDivision of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Shengnan ZhengDivision of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Jing WangDivision of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Fengsong WangSchool of Life Sciences, Anhui Medical University, Hefei, Anhui 230027, China.
Xinwang CaoSchool of Life Sciences, Anhui Medical University, Hefei, Anhui 230027, China.
Xuebiao YaoDivision of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Fan ZhengDivision of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Chuanhai FuDivision of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
University of Science and Technology of China · CNAnhui Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microtubule biogenesis initiates at various intracellular sites, including the centrosome, the Golgi apparatus, the nuclear envelope, and preexisting microtubules. Similarly, in the fission yeast Schizosaccharomyces pombe, interphase microtubules are nucleated at the spindle pole body (SPB), the nuclear envelope, and preexisting microtubules, depending on Mto1 activity. Despite the essential role of Mto1 in promoting microtubule nucleation, how distribution of Mto1 in different sites is regulated has remained elusive. Here, we show that the J-domain cochaperone Rsp1 interacts with Mto1 and specifies the localization of Mto1 to non-SPB nucleation sites. The absence of Rsp1 abolishes the localization of Mto1 to non-SPB nucleation sites, with concomitant enrichment of Mto1 to the SPB and the nuclear envelope. In contrast, Rsp1 overexpression impairs the localization of Mto1 to all microtubule organization sites. These findings delineate a previously uncharacterized mechanism in which Rsp1-Mto1 interaction orchestrates non-SPB microtubule formation.

Indexed as

Carrier ProteinsCentrosomeMicrotubule-Associated ProteinsMicrotubulesModels, BiologicalProtein BindingProtein TransportSchizosaccharomycesSchizosaccharomyces pombe ProteinsSpindle Pole BodiesCarrier ProteinsMicrotubule-Associated ProteinsMto1 protein, S pombeRsp1 protein, S pombeSchizosaccharomyces pombe Proteins

Identifiers

PMID30427751
PMCPMC6589567
OpenAlexW2900787989

What OpenQuestion holds

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LicenceCC BY-NC-SA
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.