Evidence map›Paper›PMID 30426430›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift.

Karen A Lewis, Sarah E Altschuler, Deborah S Wuttke

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. The LARP6 La module fromRNA biology · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Karen A LewisDepartment of Chemistry and Biochemistry, Texas State University, San Marcos, TX, USA.
Sarah E AltschulerDepartment of Biochemistry, UCB 596, University of Colorado Boulder, Boulder, CO, USA.
Deborah S WuttkeDepartment of Biochemistry, UCB 596, University of Colorado Boulder, Boulder, CO, USA. Deborah.Wuttke@colorado.edu.
University of Colorado Boulder · USTexas State University · US

Funding

Predoctoral Training Molecular BiophysicsT32GM065103 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI FALKE, JOSEPH J · 2002 to 2021
$4.3M
SINGLE STRANDED DNA RECOGNITION IN TELOMERESR01GM059414 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI WUTTKE, DEBORAH S. · 1999 to 2015
$3.7M
Molecular Mechanisms of the Posttranscriptional Regulator LARP6R15GM119096 · NIGMS · TEXAS STATE UNIVERSITY · PI LEWIS, KAREN ADELL · 2016 to 2019
$891k
In Vitro Reconstitution and Biochemical Characterization of Yeast TelomeraseF32GM093528 · NIGMS · UNIVERSITY OF COLORADO · PI LEWIS, KAREN ADELL · 2011 to 2012
$105k
NIGMS NIH HHS F32 GM093528NIGMS NIH HHS R01 GM059414NIGMS NIH HHS R15 GM119096NIGMS NIH HHS T32 GM065103
6 · The paper itself

Abstract

Measuring protein/DNA interactions that have apparent binding affinity constants in the low-picomolar range presents a unique experimental challenge. To probe the sequence specificity of telomere binding proteins, our laboratory has developed an electrophoretic mobility shift assay protocol that allows for the routine measurement of K

Indexed as

Acrylic ResinsAnimalsDNA-Binding ProteinsDNA, Single-StrandedElectrophoretic Mobility Shift AssayHumansProtein BindingAcrylic ResinsDNA-Binding ProteinsDNA, Single-Strandedpolyacrylamide gelsBinding assayDNA binding proteinEMSAHigh-affinity bindingNative gel

Identifiers

PMID30426430
PMCPMC6557633
OpenAlexW2901851846

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.