Evidence map›Paper›PMID 30426312›Full record

ArticleMikrochimica acta2018

Immunoelectrochemical detection of the human epidermal growth factor receptor 2 (HER2) via gold nanoparticle-based rolling circle amplification.

Congcong Shen, Shuping Liu, Xiaoqing Li, Dan Zhao, Minghui Yang

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 42 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Congcong ShenKey Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
Shuping LiuKey Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
Xiaoqing LiKey Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
Dan ZhaoKey Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
Minghui YangKey Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China. yangminghui@csu.edu.cn.ORCID 0000-0002-7612-8137
Central South University · CN

Funding

Hunan Provincial Science and Technology Plan Project 2016TP1007National Key Basic Research Program of China 2014CB744502National Natural Science Foundation of China 21575165
6 · The paper itself

Abstract

The authors describe an adapted rolling circle amplification (RCA) method for the determination of human epidermal growth factor receptor 2 (HER2). This method (which is termed immunoRCA) combines an immunoreaction with DNA based signal amplification. Gold nanoparticles (AuNPs) were loaded with antibodies against HER2 and DNA, and then fulfill the functions of recognizing HER2 and achieving signal amplification. The DNA serves as a primer to trigger RCA. This results in formation of a long DNA containing hundreds of copies of circular DNA sequence on the electrode surface. Then, molybdate is added which reacts with the phosphate group of the long DNA to generate the redox-active molybdophosphate. This, in turn, results in an increased current and, thus, in strongly increased sensitivity of the immunoassay. A linear response is linear relationship between the change of current intensity and the logarithm of the concentration in the range from 1 to 200 pg·mL

Indexed as

Nucleic Acid Amplification TechniquesAmino Acid SequenceCell Line, TumorElectrochemistryErb-b2 Receptor Tyrosine KinasesFeasibility StudiesGoldHumansImmunoassayLimit of DetectionMetal NanoparticlesOligopeptidesERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesGoldOligopeptidesHuman epidermal growth factor receptor 2ImmunoRCAMolybdateMolybdophosphateRolling circle amplification

Identifiers

PMID30426312
OpenAlexW2900484955

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.