Evidence map›Paper›PMID 30425307›Full record

ArticleScientific reports2018

Luminescence lifetime encoding in time-domain flow cytometry.

Daniel Kage, Katrin Hoffmann, Marc Wittkamp, Jens Ameskamp, Wolfgang Göhde, Ute Resch-Genger

Abstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Review
  2. Development of Time-Resolved Luminescence Measurement Instruments for Biosensing and Bioimaging - An Overview.Measurement : journal of the International Measurement Confederation · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Lab-on-Microsphere-FRET-Based Multiplex Sensor Platform.Nanomaterials (Basel, Switzerland) · 2021
    Article
  10. FRET-Based Analysis of AgInSNanomaterials (Basel, Switzerland) · 2020
    Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Daniel KageFederal Institute for Materials Research and Testing (BAM), Biophotonics Division 1.2, Richard-Willstätter-Str. 11, D-12489, Berlin, Germany.
Katrin HoffmannFederal Institute for Materials Research and Testing (BAM), Biophotonics Division 1.2, Richard-Willstätter-Str. 11, D-12489, Berlin, Germany.
Marc WittkampQuantum Analysis GmbH, Mendelstraße 17, D-48149, Münster, Germany.
Jens AmeskampQuantum Analysis GmbH, Mendelstraße 17, D-48149, Münster, Germany.
Wolfgang GöhdeQuantum Analysis GmbH, Mendelstraße 17, D-48149, Münster, Germany.
Ute Resch-GengerFederal Institute for Materials Research and Testing (BAM), Biophotonics Division 1.2, Richard-Willstätter-Str. 11, D-12489, Berlin, Germany. ute.resch@bam.de.

Funding

Bundesministerium für Bildung und Forschung (Federal Ministry of Education and Research) 13N13357Deutsche Forschungsgemeinschaft (German Research Foundation) M-era-Net ICENAP
6 · The paper itself

Abstract

Time-resolved flow cytometry represents an alternative to commonly applied spectral or intensity multiplexing in bioanalytics. At present, the vast majority of the reports on this topic focuses on phase-domain techniques and specific applications. In this report, we present a flow cytometry platform with time-resolved detection based on a compact setup and straightforward time-domain measurements utilizing lifetime-encoded beads with lifetimes in the nanosecond range. We provide general assessment of time-domain flow cytometry and discuss the concept of this platform to address achievable resolution limits, data analysis, and requirements on suitable encoding dyes. Experimental data are complemented by numerical calculations on photon count numbers and impact of noise and measurement time on the obtained lifetime values.

Indexed as

LuminescenceFlow CytometryModels, TheoreticalPhotonsSignal-To-Noise RatioTime Factors

Identifiers

PMID30425307
PMCPMC6233182

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.