ArticleScientific reports2018
Efficient silica synthesis from tetra(glycerol)orthosilicate with cathepsin- and silicatein-like proteins.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Co-option of Lysosomal Machinery for Sponge Biosilicification.bioRxiv : the preprint server for biology · 2026Article
- Directed Evolution of Silicatein Reveals Biomineralization Synergism between Protein Sequences.ACS omega · 2025Article
- Silica-Biomacromolecule Interactions: Toward a Mechanistic Understanding of Silicification.Biomacromolecules · 2025Review
- Engineered bacteria that self-assemble bioglass polysilicate coatings display enhanced light focusing.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Understanding Biomineralization Mechanisms to Produce Size-Controlled, Tailored Nanocrystals for Optoelectronic and Catalytic Applications: A Review.ACS applied nano materials · 2024Review
- Silactins and Structural Diversity of Biosilica in Sponges.Biomimetics (Basel, Switzerland) · 2024Article
- Engineered bacteria that self-assemble "bioglass" polysilicate coatings display enhanced light focusing.bioRxiv : the preprint server for biology · 2024Article
- Nanoengineered Silica-Based Biomaterials for Regenerative Medicine.International journal of molecular sciences · 2024Review
- Surface-displayed silicatein-α enzyme in bioengineeredFrontiers in systems biology · 2024Article
- Biomimetic Sol-Gel Chemistry to Tailor Structure, Properties, and Functionality of Bionanocomposites by Biopolymers and Cells.Materials (Basel, Switzerland) · 2023Review
- The genome of the reef-building glass spongeRoyal Society open science · 2023Article
- Arrested in Glass: Actin within Sophisticated Architectures of Biosilica in Sponges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Calcite Nanotuned Chitinous Skeletons of GiantInternational journal of molecular sciences · 2021Article
- Fabrication of silica on chitin in ambient conditions using silicatein fused with a chitin-binding domain.Bioprocess and biosystems engineering · 2021Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Silicateins play a key role in biosynthesis of spicules in marine sponges; they are also capable to catalyze formation of amorphous silica in vitro. Silicateins are highly homologous to cathepsins L - a family of cysteine proteases. Molecular mechanisms of silicatein activity remain controversial. Here site-directed mutagenesis was used to clarify significance of selected residues in silica polymerization. A number of mutations were introduced into two sponge proteins - silicatein A1 and cathepsin L from Latrunculia oparinae, as well as into human cathepsin L. First direction was alanine scanning of the proposed catalytic residues. Also, reciprocal mutations were introduced at selected positions that differ between cathepsins L and silicateins. Surprisingly, all the wild type and mutant proteins were capable to catalyze amorphous silica formation with a water-soluble silica precursor tetra(glycerol)orthosilicate. Some mutants possessed several-fold enhanced silica-forming activity and can potentially be useful for nanomaterial synthesis applications. Our findings contradict to the previously suggested mechanisms of silicatein action via a catalytic triad analogous to that in cathepsins L. Instead, a surface-templated biosilification by silicateins and related proteins can be proposed.
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Registered trials
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