ArticleScientific reports2018
Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Aptamer-Based Chromatographic Methods for Efficient and Economical Separation of Leukocyte Populations.ACS biomaterials science & engineering · 2023Article
- Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation.Scientific reports · 2022Article
- Natural killer cell detection, quantification, and subpopulation identification on paper microfluidic cell chromatography using smartphone-based machine learning classification.Biosensors & bioelectronics · 2022Article
- Comparison of Three CD3-Specific Separation Methods Leading to Labeled and Label-Free T Cells.Cells · 2021Article
- Expamers: a new technology to control T cell activation.Scientific reports · 2020Article
- FACS-Based Proteomics Enables Profiling of Proteins in Rare Cell Populations.International journal of molecular sciences · 2020Article
- Past, Present, and Future of Affinity-based Cell Separation Technologies.Acta biomaterialia · 2020Review
Corrections and comments
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Authors and funding
11 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells.
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Registered trials
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