Evidence map›Paper›PMID 30424792›Full record

ArticleReproductive biology and endocrinology : RB&E2018

Spatio-temporal expression profile of matrix metalloproteinase (Mmp) modulators Reck and Sparc during the rat ovarian dynamics.

Gabriel Levin, Tatiane Maldonado Coelho, Nathali Guimarães Nóbrega, Marina Trombetta-Lima, Mari Cleide Sogayar, Ana Claudia Oliveira Carreira

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive biology and endocrinology : RB&E, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Gabriel LevinNUCEL (Cell and Molecular Therapy Center), Internal Medicine Department, Medical School, University of São Paulo, Rua Pangaré, 100, Cidade Universitária, São Paulo, SP, 05360-130, Brazil.
Tatiane Maldonado CoelhoNUCEL (Cell and Molecular Therapy Center), Internal Medicine Department, Medical School, University of São Paulo, Rua Pangaré, 100, Cidade Universitária, São Paulo, SP, 05360-130, Brazil.
Nathali Guimarães NóbregaNUCEL (Cell and Molecular Therapy Center), Internal Medicine Department, Medical School, University of São Paulo, Rua Pangaré, 100, Cidade Universitária, São Paulo, SP, 05360-130, Brazil.
Marina Trombetta-LimaNUCEL (Cell and Molecular Therapy Center), Internal Medicine Department, Medical School, University of São Paulo, Rua Pangaré, 100, Cidade Universitária, São Paulo, SP, 05360-130, Brazil.
Mari Cleide SogayarNUCEL (Cell and Molecular Therapy Center), Internal Medicine Department, Medical School, University of São Paulo, Rua Pangaré, 100, Cidade Universitária, São Paulo, SP, 05360-130, Brazil.
Ana Claudia Oliveira CarreiraNUCEL (Cell and Molecular Therapy Center), Internal Medicine Department, Medical School, University of São Paulo, Rua Pangaré, 100, Cidade Universitária, São Paulo, SP, 05360-130, Brazil. ancoc@iq.usp.br.ORCID http://orcid.org/0000-0002-9142-1155
Universidade de São Paulo · BR

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2013/18842-8
6 · The paper itself

Abstract

backgroundMatrix metalloproteinases (Mmps) and their tissue inhibitors (Timps) are widely recognized as crucial factors for extracellular matrix remodeling in the ovary and are involved in follicular growth, ovulation, luteinization, and luteolysis during the estrous cycle. Recently, several genes have been associated to the modulation of Mmps activity, including Basigin (Bsg), which induces the expression of Mmps in rat ovaries; Sparc, a TGF-β modulator that is related to increased expression of Mmps in cancer; and Reck, which is associated with Mmps inhibition. However, the expression pattern of Mmp modulators in ovary dynamics is still largely uncharacterized.

methodsTo characterize the expression pattern of Mmps network members in ovary dynamics, we analyzed the spatio-temporal expression pattern of Reck and Sparc, as well as of Mmp2, Mmp9 and Mmp14 proteins, by immunohistochemistry (IHC), in pre-pubertal rat ovaries obtained from an artificial cycle induced by eCG/hCG, in the different phases of the hormone-induced estrous cycle. We also determined the gene expression profiles of Mmps (2, 9, 13 14), Timps (1, 2, 3), Sparc, Bsg, and Reck to complement this panel.

resultsIHC analysis revealed that Mmp protein expression peaks at the early stages of folliculogenesis and ovulation, decreases during ovulation-luteogenesis transition and luteogenesis, increasing again during corpus luteum maintenance and luteolysis. The protein expression patterns of these metalloproteinases and Sparc were inverse relative to the pattern displayed by Reck. We observed that the gene expression peaks of Mmps inhibitors Reck and Timp2 were closely paraleled by Mmp2 and Mmp9 suppression. The opposite was also true: increased Mmp2 and Mmp9 expression was concomitant to reduced Reck and Timp2 levels.

conclusionTherefore, our results generate a spatio-temporal expression profile panel of Mmps and their regulators, suggesting that Reck and Sparc seem to play a role during ovarian dynamics: Reck as a possible inhibitor and Sparc as an inducer of Mmps.

Indexed as

Gene Expression ProfilingAnimalsBasiginBlood ProteinsEstrous CycleFemaleGPI-Linked ProteinsMatrix MetalloproteinasesOsteonectinOvaryOvulationRats, Sprague-DawleySexual MaturationTime FactorsTissue Inhibitor of MetalloproteinasesTumor Suppressor ProteinsBasiginBlood ProteinsBsg protein, ratGPI-Linked ProteinsMatrix MetalloproteinasesOsteonectinReck protein, ratTissue Inhibitor of MetalloproteinasesTumor Suppressor ProteinsExtracellular matrixFolliculogenesisMmps systemOvary remodelingReckSparc

Identifiers

PMID30424792
PMCPMC6234678
OpenAlexW2901186814

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.