ArticleMicromachines2018
New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer.
Article in Micromachines, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Lateral Deformation of Human Red Blood Cells by Optical Tweezers.Micromachines · 2021Article
- Determining Trap Compliances, Microsphere Size Variations, and Response Linearities in Single DNA Molecule Elasticity Measurements with Optical Tweezers.Frontiers in molecular biosciences · 2021Article
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Authors and funding
4 authors.
Funding
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Abstract
We propose a new approach to calculate the sensitivity factor of the detector in optical tweezers. In this work, we used a charge-coupled device (CCD) camera and a quadrant photodiode (QPD) for the extraction of the various positions occupied by the trapped object (in this case, silica beads of different diameters). Image-J software and the Boltzmann statistical method were then used to estimate the sensitivity of the detector. Silica beads of diameter 0.8 µm, 2 µm, a system of 2 µm bead stuck to 4.5 µm one and another system of 2 µm beads stuck to 2 µm one, were studied. This work contributes significantly to making better calibration of the detector without taking into account the geometry of the object imprisoned in the optical trap. We further developed an approach to calculate the interaction force between two microbeads. This approach does not require any knowledge of solvent viscosity and works for all types of samples.
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