ArticleMicromachines2018
The Development of an Effective Bacterial Single-Cell Lysis Method Suitable for Whole Genome Amplification in Microfluidic Platforms.
Article in Micromachines, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Comparative Evaluation of Rapid Nucleic Acids Extraction Methods for Biosensor-Based Point-of-Care Solutions.Biosensors · 2026Article
- Revealing the Viable Microbial Community of Biofilm in a Sewage Treatment System Using Propidium Monoazide Combined with Real-Time PCR and Metagenomics.Microorganisms · 2024Article
- Review
- Recent Advances in Bacterial Persistence Mechanisms.International journal of molecular sciences · 2023Review
- AssesSments ofFrontiers in microbiology · 2023Article
- Article
- A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Non-random genetic alterations in the cyanobacterium Nostoc sp. exposed to space conditions.Scientific reports · 2022Article
- Whole genome sequencing of cyanobacteriumiScience · 2022Article
- Space omics research in Europe: Contributions, geographical distribution and ESA member state funding schemes.iScience · 2022Review
- Amplification of Femtograms of Bacterial DNA Within 3 h Using a Digital Microfluidics Platform for MinION Sequencing.ACS omega · 2021Article
- Linking plasmid-based beta-lactamases to their bacterial hosts using single-cell fusion PCR.eLife · 2021Article
- Review of Microfluidic Methods for Cellular Lysis.Micromachines · 2021Review
- Novel perspective on a conventional technique: Impact of ultra-low temperature on bacterial viability and protein extraction.PloS one · 2021Article
- Impact of Preanalytical and Analytical Methods on Cell-Free DNA Diagnostics.Frontiers in cell and developmental biology · 2021Article
- Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics.Microfluidics and nanofluidics · 2021Review
- An Innovative Protocol for Metaproteomic Analyses of Microbial Pathogens in Cystic Fibrosis Sputum.Frontiers in cellular and infection microbiology · 2021Article
- Bacterial Single Cell Whole Transcriptome Amplification in Microfluidic Platform Shows Putative Gene Expression Heterogeneity.Analytical chemistry · 2019Article
- Whole genome amplification of single epithelial cells dissociated from snap-frozen tissue samples in microfluidic platform.Biomicrofluidics · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Single-cell sequencing is a powerful technology that provides the capability of analyzing a single cell within a population. This technology is mostly coupled with microfluidic systems for controlled cell manipulation and precise fluid handling to shed light on the genomes of a wide range of cells. So far, single-cell sequencing has been focused mostly on human cells due to the ease of lysing the cells for genome amplification. The major challenges that bacterial species pose to genome amplification from single cells include the rigid bacterial cell walls and the need for an effective lysis protocol compatible with microfluidic platforms. In this work, we present a lysis protocol that can be used to extract genomic DNA from both gram-positive and gram-negative species without interfering with the amplification chemistry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.