ArticleInternational journal of molecular sciences2018
One Major Challenge of Sequencing Large Plant Genomes Is to Know How Big They Really Are.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- A chromosome-scale genome assembly of Hordeum erectifolium: genomic, transcriptomic and anatomical adaptations to drought in a wild barley relative.The New phytologist · 2026Article
- Epigenome and interactome profiling uncovers principles of distal regulation in the barley genome.Cell genomics · 2026Article
- The genetic mechanism of B chromosome drive in rye illuminated by chromosome-scale assembly.Nature communications · 2024Article
- Highly divergent satellitomes of two barley species of agronomic importance, Hordeum chilense and H. vulgare.Plant molecular biology · 2024Article
- Towards the Investigation of the Adaptive Divergence in a Species of Exceptional Ecological Plasticity: Chromosome-Scale Genome Assembly ofInternational journal of molecular sciences · 2023Article
- Helical coiling of metaphase chromatids.Nucleic acids research · 2023Article
- Occurrence of triploids in oil palm and their origin.Annals of botany · 2023Article
- The Use of Flow Cytometry for Estimating Genome Sizes and DNA Ploidy Levels in Plants.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Reference standards for flow cytometric estimation of absolute nuclear DNA content in plants.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Review
- Prospects of telomere-to-telomere assembly in barley: Analysis of sequence gaps in the MorexV3 reference genome.Plant biotechnology journal · 2022Article
- Fine structure and transcription dynamics of bread wheat ribosomal DNA loci deciphered by a multi-omics approach.The plant genome · 2022Article
- Novel Approaches for Species Concepts and Delimitation in Polyploids and Hybrids.Plants (Basel, Switzerland) · 2022Review
- De Novo Genome Assembly of the Japanese Wheat Cultivar Norin 61 Highlights Functional Variation in Flowering Time and Fusarium-Resistant Genes in East Asian Genotypes.Plant & cell physiology · 2021Article
- To Be or Not to Be Expressed: The First Evidence of a Nucleolar Dominance Tissue-Specificity inFrontiers in plant science · 2021Article
- A high-quality genome assembly from a single, field-collected spotted lanternfly (Lycorma delicatula) using the PacBio Sequel II system.GigaScience · 2019Article
- Towards the conservation of the Mesozoic relict fern Christensenia: a fern species with extremely small populations in China.Journal of plant research · 2019Article
- The Dark Matter of Large Cereal Genomes: Long Tandem Repeats.International journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Any project seeking to deliver a plant or animal reference genome sequence must address the question as to the completeness of the assembly. Given the complexity introduced particularly by the presence of sequence redundancy, a problem which is especially acute in polyploid genomes, this question is not an easy one to answer. One approach is to use the sequence data, along with the appropriate computational tools, the other is to compare the estimate of genome size with an experimentally measured mass of nuclear DNA. The latter requires a reference standard in order to provide a robust relationship between the two independent measurements of genome size. Here, the proposal is to choose the human male leucocyte genome for this standard: its 1C DNA amount (the amount of DNA contained within unreplicated haploid chromosome set) of 3.50 pg is equivalent to a genome length of 3.423 Gbp, a size which is just 5% longer than predicted by the most current human genome assembly. Adopting this standard, this paper assesses the completeness of the reference genome assemblies of the leading cereal crops species wheat, barley and rye.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.