Evidence map›Paper›PMID 30423842›Full record

ArticleSensors (Basel, Switzerland)2018

Development of a Protein Microarray Chip with Enhanced Fluorescence for Identification of Semen and Vaginal Fluid.

Naseem Abbas, Xun Lu, Mohsin Ali Badshah, Jung Bin In, Won Il Heo, Kui Young Park, Mi-Kyung Lee, Cho Hee Kim, Pilwon Kang, Woo-Jin Chang and 2 more

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Review
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  8. Review
  9. Interpol review of forensic biology and forensic DNA typing 2016-2019.Forensic science international. Synergy · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Naseem AbbasDepartment of Mechanical Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Korea. naseem@cau.ac.kr.
Xun LuDepartment of Mechanical Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Korea. luxun@cau.ac.kr.
Mohsin Ali BadshahDepartment of Mechanical Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Korea. mohsinali@cau.ac.kr.
Jung Bin InDepartment of Mechanical Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Korea. jbin@cau.ac.kr.
Won Il HeoDepartment of Dermatology, Chung-Ang University Hospital, 102 Heukseok-ro, Dongjak-gu, Seoul 06973, Korea. one1x@cau.ac.kr.
Kui Young ParkDepartment of Dermatology, Chung-Ang University Hospital, 102 Heukseok-ro, Dongjak-gu, Seoul 06973, Korea. kyky@caumc.or.kr.
Mi-Kyung LeeDepartment of Laboratory Medicine, Chung-Ang University Hospital, 102 Heukseok-ro, Dongjak-gu, Seoul 06973, Korea. cpworld@cau.ac.kr.
Cho Hee KimForensic DNA Division, National Forensic Service, 10 Ipchun-ro, Wonju-si, Gangwon-do 26460, Korea. chkim1220@korea.kr.
Pilwon KangForensic DNA Division, National Forensic Service, 10 Ipchun-ro, Wonju-si, Gangwon-do 26460, Korea. kpwon7@korea.kr.
Woo-Jin ChangDepartment of Mechanical Engineering, University of Wisconsin-Milwaukee, 3200 N Cramer St, Milwaukee, WI 53211, USA. wjchang@uwm.edu.
Seok-Min KimDepartment of Mechanical Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Korea. smkim@cau.ac.kr.
Seong Jun SeoDepartment of Dermatology, Chung-Ang University Hospital, 102 Heukseok-ro, Dongjak-gu, Seoul 06973, Korea. drseo@cau.ac.kr.

Funding

National Forensic Service NFS2018DNA05
6 · The paper itself

Abstract

The detection of body fluids has been used to identify a suspect and build a criminal case. As the amount of evidence collected at a crime site is limited, a multiplex identification system for body fluids using a small amount of sample is required. In this study, we proposed a multiplex detection platform using an Ag vertical nanorod metal enhanced fluorescence (MEF) substrate for semen and vaginal fluid (VF), which are important evidence in cases of sexual crime. The Ag nanorod MEF substrate with a length of 500 nm was fabricated by glancing angle deposition, and amino functionalization was conducted to improve binding ability. The effect of incubation time was analyzed, and an incubation time of 60 min was selected, at which the fluorescence signal was saturated. To assess the performance of the developed identification chip, the identification of semen and VF was carried out. The developed sensor could selectively identify semen and VF without any cross-reactivity. The limit of detection of the fabricated microarray chip was 10 times better than the commercially available rapid stain identification (RSID) Semen kit.

Indexed as

Body FluidsFemaleFluorescenceHumansMaleNanotubesOligonucleotide Array Sequence AnalysisProtein Array AnalysisSemenSemen AnalysisVaginaAg nanorodbody fluid identificationglancing angle depositionmetal-enhanced fluorescencemicroarray analysissemenvaginal fluid

Identifiers

PMID30423842
PMCPMC6263525

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.