ArticlePLoS pathogens2018
Whole genome screen reveals a novel relationship between Wolbachia levels and Drosophila host translation.
Article in PLoS pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 59 citations in OpenAlex.
- Pervasive effects ofbioRxiv : the preprint server for biology · 2026Article
- Cycloheximide, a specific inhibitor of eukaryotic protein synthesis, enhances infection of mosquito cells with Wolbachia.In vitro cellular & developmental biology. Animal · 2026Article
- Complete genome sequence ofMicrobiology resource announcements · 2025Article
- Wbm0152, an outer membrane lipoprotein of the Wolbachia endosymbiont of Brugia malayi, inhibits yeast ESCRT complex activity.PLoS pathogens · 2025Article
- Generation of Monoclonal Cultures from Wolbachia-infected Drosophila melanogaster JW18 Cell Line.Journal of visualized experiments : JoVE · 2025Article
- Article
- Longitudinal dynamics of intestinal bacteria in the life cycle and their effects on growth and development of potato tuber moth.Frontiers in microbiology · 2025Article
- Pathogen infection alters the gene expression landscape of transposable elements in Drosophila melanogaster.G3 (Bethesda, Md.) · 2024Article
- Mixed Wolbachia infections resolve rapidly during in vitro evolution.PLoS pathogens · 2024Article
- Comparative analysis of Wolbachia maternal transmission and localization in host ovaries.Communications biology · 2024Article
- Comparative analysis ofbioRxiv : the preprint server for biology · 2024Article
- Non-ovarianmicroPublication biology · 2024Article
- A Light in the Dark: Uncovering Wolbachia-Host Interactions Using Fluorescence Imaging.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Cross-validation of chemical and genetic disruption approaches to inform host cellular effects onFrontiers in microbiology · 2024Article
- The cellular lives of Wolbachia.Nature reviews. Microbiology · 2023Review
- Jamestown Canyon virus is transmissible by Aedes aegypti and is only moderately blocked by Wolbachia co-infection.PLoS neglected tropical diseases · 2023Article
- Distinct Wolbachia localization patterns in oocytes of diverse host species reveal multiple strategies of maternal transmission.Genetics · 2023Article
- Ovarian transcriptional response to Wolbachia infection in D. melanogaster in the context of between-genotype variation in gene expression.G3 (Bethesda, Md.) · 2023Article
- Article
- The Involvement of Atlastin in Dengue Virus andMicrobiology spectrum · 2022Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Wolbachia is an intracellular bacterium that infects a remarkable range of insect hosts. Insects such as mosquitos act as vectors for many devastating human viruses such as Dengue, West Nile, and Zika. Remarkably, Wolbachia infection provides insect hosts with resistance to many arboviruses thereby rendering the insects ineffective as vectors. To utilize Wolbachia effectively as a tool against vector-borne viruses a better understanding of the host-Wolbachia relationship is needed. To investigate Wolbachia-insect interactions we used the Wolbachia/Drosophila model that provides a genetically tractable system for studying host-pathogen interactions. We coupled genome-wide RNAi screening with a novel high-throughput fluorescence in situ hybridization (FISH) assay to detect changes in Wolbachia levels in a Wolbachia-infected Drosophila cell line JW18. 1117 genes altered Wolbachia levels when knocked down by RNAi of which 329 genes increased and 788 genes decreased the level of Wolbachia. Validation of hits included in depth secondary screening using in vitro RNAi, Drosophila mutants, and Wolbachia-detection by DNA qPCR. A diverse set of host gene networks was identified to regulate Wolbachia levels and unexpectedly revealed that perturbations of host translation components such as the ribosome and translation initiation factors results in increased Wolbachia levels both in vitro using RNAi and in vivo using mutants and a chemical-based translation inhibition assay. This work provides evidence for Wolbachia-host translation interaction and strengthens our general understanding of the Wolbachia-host intracellular relationship.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.