ArticleJournal of the American Society for Mass Spectrometry2019
LC-MS/MS-Based Separation and Quantification of Marfey's Reagent Derivatized Proteinogenic Amino Acid DL-Stereoisomers.
Article in Journal of the American Society for Mass Spectrometry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Bacterial Proteomics and Antibiotic Resistance Identification: Is Single-Cell Analysis a Worthwhile Pursuit?Pathogens (Basel, Switzerland) · 2025Review
- d-amino acids: new functional insights.The FEBS journal · 2025Review
- Engineering the TCA cycle regulator GarA to increase erythromycin production inMicrobiology (Reading, England) · 2025Article
- A glycosylated lipooctapeptide promotes uptake and growth of Mycobacterium abscessus in the host.Nature communications · 2025Article
- Advances in Chiral Metabolomic Profiling and Biomarker Discovery.Methods in molecular biology (Clifton, N.J.) · 2025Review
- The EpiphyteBiomolecules · 2024Article
- Discovery of Uncommon Tryptophan-Containing Diketopiperazines fromJournal of natural products · 2024Article
- High-throughput identification of gut microbiome-dependent metabolites.Nature protocols · 2024Review
- Stereoselective Amine-omics Using Heavy Atom Isotope Labeled l- and d-Marfey's Reagents.Journal of the American Society for Mass Spectrometry · 2024Article
- Biosynthesis of Macrocyclic Peptides with C-Terminal β-Amino-α-keto Acid Groups by Three Different Metalloenzymes.ACS central science · 2024Article
- Facile Method for Determining Lanthipeptide Stereochemistry.Analytical chemistry · 2024Article
- Biosynthesis of macrocyclic peptides with C-terminal β-amino-α-keto acid groups by three different metalloenzymes.bioRxiv : the preprint server for biology · 2024Article
- UHPLC-QQQ-MS/MS method for the simultaneous quantification of 18 amino acids in various meats.Frontiers in nutrition · 2024Article
- Madurastatins with Imidazolidinone Rings: Natural Products or Side-Reaction Products from Extraction Solvents?International journal of molecular sciences · 2023Article
- CHRISTMAS: Chiral Pair Isobaric Labeling Strategy for Multiplexed Absolute Quantitation of Enantiomeric Amino Acids.Analytical chemistry · 2023Article
- Analysis of D-amino acids secreted from murine islets of Langerhans using Marfey's reagent and reversed phase LC-MS/MS.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2023Article
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3 authors.
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Abstract
D-Amino acids are important biological molecules. Improved analytical methods for their resolution and quantification remain of keen interest. In this study, we investigated the use of Marfey's reagent (chiral) derivatization coupled with LC-MS/MS-based separation and detection of the resulting diastereomers for quantification of the 19 common L- and D-amino acids and glycine. Standard formic acid (pH 2)-based separations on reverse phase media were unable to separate all 19 amino acid DL pairs. In contrast, a water/acetonitrile/ammonium acetate (pH 6.5) solvent system allowed all 19 amino acid DL pairs to be chromatographically resolved on a 30 min gradient, with negative mode detection at pH 6.5 giving good sensitivity. Derivatization reaction rates between amino acids varied substantially, with overnight derivatization required for some amino acids. Chromatography at pH 6.5 combined with MS/MS quantification in negative mode demonstrated good linearity over a wide concentration range for all 20 amino acids. Matrix effects, assessed with an MRSA extract, were negligible. Marfey's derivatized analytes were stable for 24 h at room temperature. This method was demonstrated by determining the levels of these analytes in mid-log phase MRSA extracts. This approach provides for the chromatographic resolution and MS/MS-based quantification of all 20 common L- and D-amino acids in complex matrices. Graphical Abstract.
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