Evidence map›Paper›PMID 30420685›Full record

ArticleNature methods2018

Fast, in vivo voltage imaging using a red fluorescent indicator.

Madhuvanthi Kannan, Ganesh Vasan, Cheng Huang, Simon Haziza, Jin Zhong Li, Hakan Inan, Mark J Schnitzer, Vincent A Pieribone

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.

0numbers the graph read from it
0cells of the map it votes in
85citing papers in PubMed
8.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

85 citing papers in PubMed, 154 citations in OpenAlex.

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25 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Madhuvanthi KannanThe John B. Pierce Laboratory, New Haven, CT, USA.ORCID http://orcid.org/0000-0003-0664-7377
Ganesh VasanThe John B. Pierce Laboratory, New Haven, CT, USA.ORCID http://orcid.org/0000-0002-6612-7739
Cheng HuangJames H. Clark Center, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-9936-4813
Simon HazizaJames H. Clark Center, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-5051-8148
Jin Zhong LiJames H. Clark Center, Stanford University, Stanford, CA, USA.
Hakan InanJames H. Clark Center, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0003-3505-8838
Mark J SchnitzerJames H. Clark Center, Stanford University, Stanford, CA, USA.
Vincent A PieriboneThe John B. Pierce Laboratory, New Haven, CT, USA. vincent.pieribone@yale.edu.ORCID http://orcid.org/0000-0002-5836-5454
Clark University · USYale University · US

Funding

Towards a Complete Description of the Circuitry Underlying Sharp Wave-Mediated Memory ReplayU19NS104590 · NINDS · STANFORD UNIVERSITY · PI SOLTESZ, IVAN · 2017 to 2021
$13.2M
High-Speed, wide field fluorescent imaging of cortex in freely moving animalsR01NS065110 · NINDS · JOHN B. PIERCE LABORATORY, INC. · PI CULURCIELLO, EUGENIO, PIERIBONE, VINCENT A · 2009 to 2015
$3.7M
High Throughput of Protein-based Voltage ProbesU01NS103517 · NINDS · JOHN B. PIERCE LABORATORY, INC. · PI PIERIBONE, VINCENT A · 2017 to 2019
$2.4M
Genetically-Encoded Voltage Probe DevelopmentR01NS083875 · NINDS · JOHN B. PIERCE LABORATORY, INC. · PI HUGHES, THOMAS E, NITABACH, MICHAEL · 2013 to 2016
$2.3M
Development of Protein-based Voltage ProbesU01NS090565 · NINDS · JOHN B. PIERCE LABORATORY, INC. · PI PIERIBONE, VINCENT A · 2014 to 2016
$1.7M
NINDS NIH HHS R01 NS065110NINDS NIH HHS R01 NS083875NINDS NIH HHS U01 NS090565NINDS NIH HHS U01 NS103517NINDS NIH HHS U19 NS104590
6 · The paper itself

Abstract

Genetically encoded voltage indicators (GEVIs) are emerging optical tools for acquiring brain-wide cell-type-specific functional data at unparalleled temporal resolution. To broaden the application of GEVIs in high-speed multispectral imaging, we used a high-throughput strategy to develop voltage-activated red neuronal activity monitor (VARNAM), a fusion of the fast Acetabularia opsin and the bright red fluorophore mRuby3. Imageable under the modest illumination intensities required by bright green probes (<50 mW mm

Indexed as

Action PotentialsAnimalsBrainCells, CulturedDrosophila melanogasterElectrophysiological PhenomenaFemaleFluorescent DyesHEK293 CellsHumansImage Processing, Computer-AssistedLuminescent ProteinsMaleMiceMice, Inbred C57BLMicroscopy, FluorescenceFluorescent DyesLuminescent ProteinsRed Fluorescent Protein

Identifiers

PMID30420685
PMCPMC6516062
OpenAlexW2899201459

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.