Evidence map›Paper›PMID 30419729›Full record

ArticleThe Korean journal of parasitology2018

Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets.

Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak and 7 more

Abstract read
In one paragraph

Article in The Korean journal of parasitology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Descriptive Comparison of ELISAs for the Detection ofPathogens (Basel, Switzerland) · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Won Gi YooDepartment of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul 06974, Korea.
Sun-Min KimDepartment of Veterinary Parasitology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Eun Jeong WonDepartment of Parasitology and Tropical Medicine, Chonnam National University Medical School, Gwangju 61469, Korea.
Ji-Yun LeeDepartment of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul 06974, Korea.
Fuhong DaiDepartment of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul 06974, Korea.
Ho Choon WooDepartment of Veterinary Parasitology, College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea.
Ho-Woo NamDepartment of Parasitology, College of Medicine, Catholic University, Seoul 06591, Korea.
Tae Im KimDepartment of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul 06974, Korea.
Jeong-Hee HanDepartment of Veterinary Pathology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea.
Dongmi KwakDepartment of Veterinary Parasitology, Kyungpook National University College of Veterinary Sciences, Daegu 41566, Korea.
Yun Sang ChoParasitic and Insect Disease Laboratory, Bacterial and Parasitic Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Seung-Won KangVexPert Co., Suwon 16614, Korea; 11KoreaBioGroup Co., Incheon 22013, Korea.
Tong-Soo KimDepartment of Parasitology and Tropical Medicine and Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon 22333, Korea.
Xing-Quan ZhuState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, P. R. China.
Chunren WangDepartment of Preventive Veterinary Medicine, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, P. R. China.
Heejeong YounDepartment of Veterinary Parasitology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Sung-Jong HongDepartment of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul 06974, Korea.

Funding

Animal and Plant Quarantine Agency Z-1541778-2013-14-01
6 · The paper itself

Abstract

To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.

Indexed as

AnimalsAntibodies, ProtozoanBiomarkersEnzyme-Linked Immunosorbent AssayFood ContaminationMeatPrevalenceRepublic of KoreaSwineSwine DiseasesToxoplasmaToxoplasmosis, AnimalAntibodies, ProtozoanBiomarkersELISAimportedKoreanporktissue fluidToxoplasma gondii

Identifiers

PMID30419729
PMCPMC6243190

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.