ArticlePLoS neglected tropical diseases2018
Comparative transcriptome analysis and RNA interference reveal CYP6A8 and SNPs related to pyrethroid resistance in Aedes albopictus.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Resistance to pyrethroids in Aedes aegypti: Insights into transcriptomic response to different insecticide concentrations.PLoS neglected tropical diseases · 2026Article
- Integrated transcriptomic and variant analysis reveals molecular mechanisms of pyrethroid resistance in a genetically homogenized Cas9 strain of Aedes aegypti.Archives of toxicology · 2026Article
- Metabolic resistance of the tiger mosquito to pyrethroid insecticides in La Réunion Island likely results from local adaptation.Genome biology and evolution · 2026Article
- Expression of cytochrome P450 monooxygenase genes in pyrethroid-resistant Aedes albopictus.Scientific reports · 2025Article
- Article
- Unveiling candidate genes for metabolic resistance to malathion in Aedes albopictus through RNA sequencing-based transcriptome profiling.PLoS neglected tropical diseases · 2024Article
- Resistance to pyrethroids and the relationship between adult resistance and knockdown resistance (kdr) mutations in Aedes albopictus in dengue surveillance areas of Guizhou Province, China.Scientific reports · 2024Article
- Heat shock proteins, thermotolerance, and insecticide resistance in mosquitoes.Frontiers in insect science · 2024Review
- In Vitro and In Vivo Validation of CYP6A14 and CYP6N6 Participation in Deltamethrin Metabolic Resistance in Aedes albopictus.The American journal of tropical medicine and hygiene · 2023Article
- The buzz in the field: the interaction between viruses, mosquitoes, and metabolism.Frontiers in cellular and infection microbiology · 2023Review
- Insecticide Susceptibility Status ofInsects · 2022Article
- Spatial heterogeneity of knockdown resistance mutations in the dengue vector Aedes albopictus in Guangzhou, China.Parasites & vectors · 2022Article
- Expansive and Diverse Phenotypic Landscape of Field Aedes aegypti (Diptera: Culicidae) Larvae with Differential Susceptibility to Temephos: Beyond Metabolic Detoxification.Journal of medical entomology · 2022Article
- Down-Regulation of P450 Genes Enhances Susceptibility to Indoxacarb and Alters Physiology and Development of Fall Armyworm,Frontiers in physiology · 2022Article
- Maternally Instigated Diapause inFrontiers in behavioral neuroscience · 2022Review
- Uncovering the Worldwide Diversity and Evolution of the Virome of the MosquitoesMicroorganisms · 2021Article
- From Global to Local-New Insights into Features of Pyrethroid Detoxification in Vector Mosquitoes.Insects · 2021Review
- The Midgut Microbiota of ColombianInsects · 2020Article
- Improved reference genome of the arboviral vector Aedes albopictus.Genome biology · 2020Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
Wide and improper application of pyrethroid insecticides for mosquito control has resulted in widespread resistance in Aedes albopictus mosquitoes, an important dengue vector. Therefore, understanding the molecular regulation of insecticide resistance is urgently needed to provide a basis for developing novel resistance diagnostic methods and vector control approaches. We investigated the transcriptional profiles of deltamethrin-resistant and -susceptible Ae. albopictus by performing paired-end sequencing for RNA expression analysis. The analysis used 24 independent libraries constructed from 12 wild-caught resistant and 12 susceptible Ae. albopictus female adults. A total of 674,503,592 and 612,512,034 reads were obtained, mapped to the Ae. albopictus genome and assembled into 20,091 Ae. albopictus transcripts. A total of 1,130 significantly differentially expressed genes included 874 up-regulated genes and 256 down-regulated genes in the deltamethrin-resistant individuals. These differentially expressed genes code for cytochrome P450s, cuticle proteins, glutathione S-transferase, serine proteases, heat shock proteins, esterase, and others. We selected three highly differentially expressed candidate genes, CYP6A8 and two genes of unknown function (CCG013931 and CCG000656), to test the association between these 3 genes and deltamethrin resistance using RNAi through microinjection in adult mosquitoes and oral feeding in larval mosquitoes. We found that expression knockdown of these three genes caused significant changes in resistance. Further, we detected 1,162 single nucleotide polymorphisms (SNPs) with a frequency difference of more than 50%. Among them, 5 SNPs in 4 cytochrome P450 gene families were found to be significantly associated with resistance in a genotype-phenotype association study using independent field-collected mosquitoes of known resistance phenotypes. Altogether, a combination of novel individually based transcriptome profiling, RNAi, and genetic association study identified both differentially expressed genes and SNPs associated with pyrethroid resistance in Ae. albopictus mosquitoes, and laid a useful foundation for further studies on insecticide resistance mechanisms.
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