ArticlePLoS genetics2018
Arabidopsis class I formins control membrane-originated actin polymerization at pollen tube tips.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A multiscale growth atlas of Arabidopsis: linking cell dynamics to organ development.The New phytologist · 2026Pooled it
- Genome-wide identification and gene expression analysis of Formin homology 2 (FH2) genes in peanut.BMC plant biology · 2026Article
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Genome-wide characterization and expression profiling of FIMBRIN gene family members in response to abiotic stress in Medicago sativa.BMC plant biology · 2025Article
- Genome-Wide Characterization and Analysis of theGenes · 2025Article
- AtFH5 recruits and transports the arabinogalactan protein AGP23 to maintain the tip growth of pollen tube.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Transmembrane formins as active cargoes of membrane trafficking.Journal of experimental botany · 2024Review
- From gametes to zygote: Mechanistic advances and emerging possibilities in plant reproduction.Plant physiology · 2024Review
- Cooperative actin filament nucleation by the Arp2/3 complex and formins maintains the homeostatic cortical array in Arabidopsis epidermal cells.The Plant cell · 2024Article
- Differential sensitivity of ADF isovariants to a pH gradient promotes pollen tube growth.The Journal of cell biology · 2023Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Activation of actin-depolymerizing factor by CDPK16-mediated phosphorylation promotes actin turnover in Arabidopsis pollen tubes.PLoS biology · 2023Article
- O-glycosylation of the extracellular domain of pollen class I formins modulates their plasma membrane mobility.Journal of experimental botany · 2022Article
- Formins control dynamics of F-actin in the central cell ofPlant signaling & behavior · 2021Article
- Comprehensive analysis of formin gene family highlights candidate genes related to pollen cytoskeleton and male fertility in wheat (Triticum aestivum L.).BMC genomics · 2021Article
- The quest for the central players governing pollen tube growth and guidance.Plant physiology · 2021Review
- Plant ProteinFrontiers in plant science · 2021Review
- TheFrontiers in plant science · 2021Article
- An Update on the Role of the Actin Cytoskeleton in Plasmodesmata: A Focus on Formins.Frontiers in plant science · 2021Review
- Kinase Partner Protein Plays a Key Role in Controlling the Speed and Shape of Pollen Tube Growth in Tomato.Plant physiology · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A population of dynamic apical actin filaments is required for rapid polarized pollen tube growth. However, the cellular mechanisms driving their assembly remain incompletely understood. It was postulated that formin is a major player in nucleating apical actin assembly, but direct genetic and cytological evidence remains to be firmly established. Here we found that both Arabidopsis formin 3 (AtFH3) and formin 5 (AtFH5) are involved in the regulation of apical actin polymerization and actin array construction in pollen tubes, with AtFH3 playing a more dominant role. We found that both formins have plasma membrane (PM) localization signals but exhibit distinct PM localization patterns in the pollen tube, and loss of their function reduces the amount of apical actin filaments. Live-cell imaging revealed that the reduction in filamentous actin is very likely due to the decrease in filament elongation. Furthermore, we found that the rate of tip-directed vesicle transport is reduced and the pattern of apical vesicle accumulation is altered in formin loss-of-function mutant pollen tubes, which explains to some extent the reduction in pollen tube elongation. Thus, we provide direct genetic and cytological evidence showing that formin is an important player in nucleating actin assembly from the PM at pollen tube tips.
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