ArticleJournal of experimental botany2019
Homology modeling and in vivo functional characterization of the zinc permeation pathway in a heavy metal P-type ATPase.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Transcriptome analysis of high- and low-selenium genotypes identifies genes responsible for selenium absorption, translocation, and accumulation.Frontiers in plant science · 2024Article
- The TabHLH35-TaWAK20-TaSPL5 pathway positively regulates Cd stress in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Identification of low grain cadmium accumulation genotypes and its physiological mechanism in maize (Zea mays L.).Environmental science and pollution research international · 2022Article
- Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis.Frontiers in plant science · 2022Article
- Recent advances in physiological and molecular mechanisms of heavy metal accumulation in plants.Environmental science and pollution research international · 2021Review
- Melatonin Confers Plant Cadmium Tolerance: An Update.International journal of molecular sciences · 2021Review
- Review
- Reducing Cadmium Accumulation in Plants: Structure-Function Relations and Tissue-Specific Operation of Transporters in the Spotlight.Plants (Basel, Switzerland) · 2020Review
- Pump Proton and Laryngeal HInternational journal of general medicine · 2020Review
- di-Cysteine Residues of theFrontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The P1B ATPase heavy metal ATPase 4 (HMA4) is responsible for zinc and cadmium translocation from roots to shoots in Arabidopsis thaliana. It couples ATP hydrolysis to cytosolic domain movements, enabling metal transport across the membrane. The detailed mechanism of metal permeation by HMA4 through the membrane remains elusive. Here, homology modeling of the HMA4 transmembrane region was conducted based on the crystal structure of a ZntA bacterial homolog. The analysis highlighted amino acids forming a metal permeation pathway, whose importance was subsequently investigated functionally through mutagenesis and complementation experiments in plants. Although the zinc pathway displayed overall conservation among the two proteins, significant differences were observed, especially in the entrance area with altered electronegativity and the presence of a ionic interaction/hydrogen bond network. The analysis also newly identified amino acids whose mutation results in total or partial loss of the protein function. In addition, comparison of zinc and cadmium accumulation in shoots of A. thaliana complemented lines revealed a number of HMA4 mutants exhibiting different abilities in zinc and cadmium translocation. These observations could be instrumental to design low cadmium-accumulating crops, hence decreasing human cadmium exposure.
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