ArticleNature biotechnology2018
ClampFISH detects individual nucleic acid molecules using click chemistry-based amplification.
Article in Nature biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 148 citations in OpenAlex.
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- The Evolution of Spatial Omics Technologies Introduces A Novel Avenue for Lung Cancer Research.Genomics, proteomics & bioinformatics · 2026Review
- Applications and development of in situ nucleic acid visualization techniques.Frontiers in bioengineering and biotechnology · 2026Review
- Tetrahedral DNA dendritic nanostructure-enhanced FISH for high-speed, sensitive spatial transcriptomics.Nature communications · 2025Article
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- HybriSeq: probe-based device-free single-cell RNA profiling.Communications biology · 2025Article
- inCu-click: DNA-enhanced ligand enables live-cell, intracellular click chemistry reaction with copper catalyst.Nature communications · 2025Article
- Deep-tissue transcriptomics and subcellular imaging at high spatial resolution.Science (New York, N.Y.) · 2025Article
- Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER.Nature methods · 2025Article
- Spatial omics advances for in situ RNA biology.Molecular cell · 2024Review
- Effect of genomic and cellular environments on gene expression noise.Genome biology · 2024Article
- Localization of RNAs to the mitochondria-mechanisms and functions.RNA (New York, N.Y.) · 2024Review
- Highly sensitive spatial transcriptomics using FISHnCHIPs of multiple co-expressed genes.Nature communications · 2024Article
- Image-based 3D genomics through chromatin tracing.Nature reviews. Methods primers · 2024Article
- Rapid in situ RNA imaging based on Cas12a thrusting strand displacement reaction.Nucleic acids research · 2023Article
- Enablers and challenges of spatial omics, a melting pot of technologies.Molecular systems biology · 2023Review
- The dawn of spatial omics.Science (New York, N.Y.) · 2023Review
- Recent Advances in High-sensitivityActa histochemica et cytochemica · 2023Review
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Methods for detecting single nucleic acids in cell and tissues, such as fluorescence in situ hybridization (FISH), are limited by relatively low signal intensity and nonspecific probe binding. Here we present click-amplifying FISH (clampFISH), a method for fluorescence detection of nucleic acids that achieves high specificity and high-gain (>400-fold) signal amplification. ClampFISH probes form a 'C' configuration upon hybridization to the sequence of interest in a double helical manner. The ends of the probes are ligated together using bio-orthogonal click chemistry, effectively locking the probes around the target. Iterative rounds of hybridization and click amplify the fluorescence intensity. We show that clampFISH enables the detection of RNA species with low-magnification microscopy and in RNA-based flow cytometry. Additionally, we show that the modular design of clampFISH probes allows multiplexing of RNA and DNA detection, that the locking mechanism prevents probe detachment in expansion microscopy, and that clampFISH can be applied in tissue samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.