ArticlePhysical chemistry chemical physics : PCCP2018
The sacrificial inactivation of the blue-light photosensor cryptochrome from Drosophila melanogaster.
Article in Physical chemistry chemical physics : PCCP, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Genetic analysis of cryptochrome in insect magnetosensitivity.Frontiers in physiology · 2022Pooled it
- Linear free energy relationship between reduction potential and photoreduction rate: studies on Drosophila cryptochrome.The FEBS journal · 2025Article
- Effect of Excitation Wavelength in Single-Molecule Photochemistry of Terrylene.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- Cryptochrome magnetoreception: Time course of photoactivation from non-equilibrium coarse-grained molecular dynamics.Computational and structural biotechnology journal · 2024Article
- A structural decryption of cryptochromes.Frontiers in chemistry · 2024Review
- Tracking the Electron Transfer Cascade in European Robin Cryptochrome 4 Mutants.Journal of the American Chemical Society · 2023Article
- Cryptochrome magnetoreception: four tryptophans could be better than three.Journal of the Royal Society, Interface · 2021Article
- Stabilisation of the Fatty Acid Decarboxylase from Chlorella variabilis by Caprylic Acid.Chembiochem : a European journal of chemical biology · 2021Article
- Tuning flavin environment to detect and control light-induced conformational switching in Drosophila cryptochrome.Communications biology · 2021Article
- Deazaflavin reductive photocatalysis involves excited semiquinone radicals.Nature communications · 2020Article
- Flavin doesn't put all oxygens in one basket.Nature chemical biology · 2020Article
- One Actor, Multiple Roles: The Performances of Cryptochrome inFrontiers in physiology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila melanogaster cryptochrome functions as the primary blue-light receptor that mediates circadian photo entrainment. Absorption of a photon leads to reduction of the protein-bound FAD via consecutive electron transfer along a conserved tryptophan tetrad resembling the signalling state required for conformational changes and induction of subsequent signalling cascades. However, how the initial photochemistry and subsequent dark processes leading to downstream signalling are linked to each other at the molecular level is still poorly understood. Here, we investigated in detail the initial photochemical events in DmCRY by time-resolved and stationary absorption spectroscopy combined with quantum chemical and molecular dynamics calculations. We resolved the early events along the conserved tryptophan tetrad and the final deprotonation of the terminal tryptophanyl radical cation. These initial events lead to conformational changes, such as the known C-terminal tail release, Trp decomposition, and finally FAD release providing evidence that DmCRY does not undergo a photocycle. We propose that light is a negative regulator of DmCRY stability even under in vitro conditions where the proteasomal machinery is missing, that is in line with its biological function, i.e. entrainment of the circadian clock.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.