ArticleJournal of visualized experiments : JoVE2018
The Unpredictable Chronic Mild Stress Protocol for Inducing Anhedonia in Mice.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Validity of chronic restraint stress for modeling anhedonic-like behavior in rodents: a systematic review and meta-analysis.The Journal of international medical research · 2022Pooled it
- Continuous OFT-Based Behavioral Phenotyping Reveals Neural Stem Cell-Associated Shifts in Depression-like Mice.Brain sciences · 2026Article
- Opioidergic modulation of stress-induced hyperalgesia in adult zebrafish.Journal of neural transmission (Vienna, Austria : 1996) · 2026Article
- Protective Effects ofPharmaceuticals (Basel, Switzerland) · 2026Article
- Comparative analysis of multiple chronic unpredictable mild stress paradigms in mice: Behavioural and physiological validity.Neurobiology of stress · 2026Article
- Stress Accelerates Depressive-Like Behaviors through Increase of Notch2 Expression in N141I Mutation Presenilin-2 Transgenic Mice.Biomolecules & therapeutics · 2026Article
- Hippocampal miR-34a/SIRT1 and BDNF Responses Following Adolescent Stress: Differential Effects of Ketamine and Fluoxetine.Turk psikiyatri dergisi = Turkish journal of psychiatry · 2026Article
- Chronic Quetiapine Administration Has a Therapeutic Effect on Anxiety and Depression-Like Behavior in a Chronic Unpredictable Mild Stress (CUMS) Rat Model.Brain and behavior · 2025Article
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- The reduction in locomotor activity induced by restraint stress in young male mice involves the downregulation of hippocampal serotonergic and dopaminergic markers.IBRO neuroscience reports · 2025Article
- Chronic Stress Modulates Microglial Activation Dynamics, Shaping Priming Responses to Subsequent Stress.Brain sciences · 2025Article
- Therapeutic potential of a rhein-loaded self-nano-emulsifying drug delivery system in ameliorating LPS-induced depression: mechanistic insights and behavioral outcomes.Frontiers in pharmacology · 2025Article
- Mechanistic Exploration of Shugan Jianpi Formula for Treating Triple-Negative Breast Cancer Under Chronic Stress: A Network Pharmacology-Guided Experimental Study.Drug design, development and therapy · 2025Article
- Jiannao Pills alleviate depression-like behavior in chronic unpredictable mild stress-induced mice through NF-κB/NLRP3 pathway.Frontiers in pharmacology · 2025Article
- Vilazodone Alleviates Neurogenesis-Induced Anxiety in the Chronic Unpredictable Mild Stress Female Rat Model: Role of Wnt/β-Catenin Signaling.Molecular neurobiology · 2024Article
- Rodent tests of depression and anxiety: Construct validity and translational relevance.Cognitive, affective & behavioral neuroscience · 2024Review
- Ketamine's rapid and sustained antidepressant effects are driven by distinct mechanisms.Cellular and molecular life sciences : CMLS · 2024Article
- Sex in aging matters: exercise and chronic stress differentially impact females and males across the lifespan.Frontiers in aging neuroscience · 2024Article
- The impact of stress on the behavior of C57BL/6 mice with liver injury: a comparative study.Frontiers in behavioral neuroscience · 2024Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Depression is a highly prevalent and debilitating condition, only partially addressed by current pharmacotherapies. The lack of response to treatment by many patients prompts the need to develop new therapeutic alternatives and to better understand the etiology of the disorder. Pre-clinical models with translational merits are rudimentary for this task. Here we present a protocol for the unpredictable chronic mild stress (UCMS) method in mice. In this protocol, adolescent mice are chronically exposed to interchanging unpredictable mild stressors. Resembling the pathogenesis of depression in humans, stress exposure during the sensitive period of mice adolescence instigates a depressive-like phenotype evident in adulthood. UCMS can be used for screenings of antidepressants on the variety of depressive-like behaviors and neuromolecular indices. Among the more prominent tests to assess depressive-like behavior in rodents is the sucrose preference test (SPT), which reflects anhedonia (core symptom of depression). The SPT will also be presented in this protocol. The ability of UCMS to induce anhedonia, instigate long-term behavioral deficits and enable reversal of these deficits via chronic (but not acute) treatment with antidepressants strengthens the protocol's validity compared to other animal protocols for inducing depressive-like behaviors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.