ArticleCellular and molecular bioengineering2018
Restoring vasculogenic potential of endothelial cells from diabetic patients through spheroid formation.
Article in Cellular and molecular bioengineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- 3D printed elastomeric biomaterial mitigates compaction during in vitro vasculogenesis.Acta biomaterialia · 2023Article
- Hydrogel degradation promotes angiogenic and regenerative potential of cell spheroids for wound healing.Materials today. Bio · 2023Article
- Engineered bone marrow as a clinically relevant ex vivo model for primary bone cancer research and drug screening.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Tuning the Microenvironment to Create Functionally Distinct Mesenchymal Stromal Cell Spheroids.Annals of biomedical engineering · 2023Article
- Effects of High Glucose on Human Endothelial Cells Exposed to Simulated Microgravity.Biomolecules · 2023Article
- Single-cell RNA-seq and bulk-seq identify RAB17 as a potential regulator of angiogenesis by human dermal microvascular endothelial cells in diabetic foot ulcers.Burns & trauma · 2023Article
- Strategies to capitalize on cell spheroid therapeutic potential for tissue repair and disease modeling.NPJ Regenerative medicine · 2022Review
- Sulfated Alginate Hydrogels Prolong the Therapeutic Potential of MSC Spheroids by Sequestering the Secretome.Advanced healthcare materials · 2021Article
- Three-Dimensional Printed Stamps for the Fabrication of Patterned Microwells and High-Throughput Production of Homogeneous Cell Spheroids.3D printing and additive manufacturing · 2020Article
- Tunable fibrin-alginate interpenetrating network hydrogels to support cell spreading and network formation.Acta biomaterialia · 2020Article
- Spatial localization of endothelial cells in heterotypic spheroids influences Notch signaling.Journal of molecular medicine (Berlin, Germany) · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
introductionDiabetes is an emerging epidemic in the developing world and represents a major risk factor for cardiovascular disease. Among other issues, patients with diabetes suffer from diminished endothelial cell (EC) function, which contributes to impaired vasculogenesis and recovery from ischemic insult. The formation of cells into three-dimensional spheroids promotes cell survival and activates key signaling pathways through the upregulation of cell-cell contacts, providing an opportunity to overcome shortcomings associated with individual autologous cells.
methodsWe hypothesized that forming human microvascular endothelial cells (HMVECs) from diabetic patients into spheroids would restore their vasculogenic potential following upregulation of these cell-cell interactions. HMVEC spheroids were formed and suspended in fibrin gels to quantify vasculogenic potential.
resultsIndividual HMVECs from diabetic patients exhibited similar proliferative and chemotactic potential to cells from healthy donors but reduced tubulogenesis. HMVEC spheroids formed from diabetic donors formed more sprouts than spheroids from healthy donors, and more sprouts than individual cells from either population.
conclusionsCompared to cells from healthy donors, sprout formation was more efficiently abrogated in HMVECs from diabetic patients by blocking matrix metalloproteinase activity. This study demonstrates a promising approach for restoring the diminished vasculogenic potential of endothelial cells in diabetic patients.
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