ArticleJournal of assisted reproduction and genetics2019
Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06261671 (Antioxidants-enriched Single-step vs Global Total Single-step Embryo Culture Media), which is not on this map. Cited by 22 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Antioxidants-enriched Single-step vs Global Total Single-step Embryo Culture Media: Effect on Blastocyst Euploidy Rates.
Who cites it
22 citing papers in PubMed, 3 syntheses or guidelines pooled it, 44 citations in OpenAlex.
- An Updated Understanding of Human Embryonic Fragmentation.Reproductive sciences (Thousand Oaks, Calif.) · 2026Pooled it
- Effect of cytoplasmic fragmentation on embryo development, quality, and pregnancy outcome: a systematic review of the literature.Reproductive biology and endocrinology : RB&E · 2024Pooled it
- Molecular Drivers of Developmental Arrest in the Human Preimplantation Embryo: A Systematic Review and Critical Analysis Leading to Mapping Future Research.International journal of molecular sciences · 2021Pooled it
- Vitamin B12 and Reproductive Health: Clinical Insights, Emerging Mechanistic Understanding, and Nutritional Aspects.Molecular reproduction and development · 2026Review
- Reactive Oxygen Species in Embryo Development: Sources, Impacts, and Implications for In Vitro Culture Systems.Life (Basel, Switzerland) · 2026Review
- Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes.Reproductive biology and endocrinology : RB&E · 2024Article
- Impact of Ovarian Factor Mediums on the Apoptotic Gene Expression and Embryo Quality Derived From Vitrified Immature Human Oocytes.Journal of obstetrics and gynaecology of India · 2023Article
- Oxidative Stress in Assisted Reproductive Techniques, with a Focus on an Underestimated Risk Factor.Current issues in molecular biology · 2023Review
- Dietary flaxseed oil and vitamin E improve semen qualityFrontiers in endocrinology · 2023Article
- The Impact ofTheScientificWorldJournal · 2023Article
- Relationship of the levels of reactive oxygen species in the fertilization medium with the outcome ofFrontiers in endocrinology · 2023Article
- Mitochondria Transfer from Adipose Stem Cells Improves the Developmental Potential of Cryopreserved Oocytes.Biomolecules · 2022Article
- Cellular and Molecular Nature of Fragmentation of Human Embryos.International journal of molecular sciences · 2022Review
- Riboceine Rescues Auranofin-Induced Craniofacial Defects in Zebrafish.Antioxidants (Basel, Switzerland) · 2021Article
- Male factor infertility and placental pathology in singleton live births conceived with in vitro fertilization.Journal of assisted reproduction and genetics · 2021Article
- Enhanced Synergistic-Antioxidant Activity of Melatonin and Tretinoin by Co-encapsulation into Amphiphilic Chitosan Nanocarriers: During Mice In Vitro Matured Oocyte/Morula-Compact Stage Embryo Culture Model.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Dietary Antioxidants in the Treatment of Male Infertility: Counteracting Oxidative Stress.Biology · 2021Review
- Distinct Signaling Pathways DistinguishFrontiers in cell and developmental biology · 2021Article
- Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes culturedVeterinary world · 2021Article
- Effects of N-Acetylcysteine on the reproductive performance, oxidative stress and RNA sequencing of Nubian goats.Veterinary medicine and science · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeReactive oxygen species (ROS) are thought to play a critical role in the success of IVF. The relationships between oxidative stress parameters in culture media and IVF outcomes have not been extensively investigated. The objective of this study is to examine the relationships between early human embryonic parameters and levels of ROS in culture media.
methodsThis prospective study was conducted with 2633 spent culture media collected from patients undergoing conventional IVF (n = 101) and intracytoplasmic sperm injection (ICSI) (n = 60). Both fertilization and early culture were performed in universal IVF medium and G series medium. ROS levels were measured by chemiluminescence assays using luminol as the probe on days 1, 3, and 5 and determined the relationships of ROS levels with zygote condition, embryo quality, and clinical pregnancy rate.
resultsROS levels per embryo in culture media on the corresponding days 1, 3, and 5 showed significant correlations between each pair in the total cohort. Similar results were observed in the IVF and ICSI groups, but day 1 and day 3 ROS levels were significantly higher in culture media of IVF than of ICSI embryos. ROS levels in culture medium were not significantly associated with embryo quality, blastocyst formation, or arrest. ROS levels on day 1 were similar in media of normally fertilized zygotes, unfertilized oocytes, and polyspermic zygotes and were not associated with delayed embryonic development, high fragmentation, blastocyst formation, or arrest after prolonged culture. ROS levels in media were not associated with the likelihood of conception.
conclusionsROS levels in culture media may not be an effective indicator of embryo selection for IVF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.