ArticleAMB Express2018
Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos.
Article in AMB Express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 89 citations in OpenAlex.
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- Dynamic multi-omics analysis reveals the correlation between aroma compounds and symbiotic microbial community during tobacco leaf aging process.BMC plant biology · 2025Article
- Variation in Microbiota and Chemical Components WithinMicroorganisms · 2025Article
- Gut microbial-derived phenylacetylglutamine accelerates host cellular senescence.Nature aging · 2025Article
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- Plant developmental stage drives the assembly and functional adaptability of endophytic microbial communities.Frontiers in microbiology · 2025Article
- Native edaphoclimatic regions shape soil communities of crop wild progenitors.ISME communications · 2025Article
- Shaping the future of tobacco through microbial insights: a review of advances and applications.Frontiers in bioengineering and biotechnology · 2025Review
- Toward Characterizing Environmental Sources of Non-tuberculous Mycobacteria (NTM) at the Species Level: A Tutorial Review of NTM Phylogeny and Phylogenetic Classification.ACS environmental Au · 2024Review
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- How the root bacterial community ofFrontiers in plant science · 2024Article
- Microbial community and chemical composition of cigar tobacco (Plant direct · 2023Article
- Host species and habitat shape fish-associated bacterial communities: phylosymbiosis between fish and their microbiome.Microbiome · 2023Article
- Primer selection impacts the evaluation of microecological patterns in environmental microbiomes.iMeta · 2023Article
- The bacterial succession and its role in flavor compounds formation during the fermentation of cigar tobacco leaves.Bioresources and bioprocessing · 2023Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
Abstract
Selection of optimal primer pairs in 16S rRNA gene sequencing is a pivotal issue in microorganism diversity analysis. However, limited effort has been put into investigation of specific primer sets for analysis of the bacterial diversity of aging flue-cured tobaccos (AFTs), as well as prediction of the function of the bacterial community. In this study, the performance of four primer pairs in determining bacterial community structure based on 16S rRNA gene sequences in AFTs was assessed, and the functions of genes were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Results revealed that the primer set 799F-1193R covering the amplification region V5V6V7 gave a more accurate picture of the bacterial community structure of AFTs, with lower co-amplification levels of chloroplast and mitochondrial genes, and more genera covered than when using the other primers. In addition, functional gene prediction suggested that the microbiome of AFTs was involved in kinds of interested pathways. A high abundance of functional genes involved in nitrogen metabolism was detected in AFTs, reflecting a high level of bacteria involved in degrading harmful nitrogen compounds and generating nitrogenous nutrients for others. Additionally, the functional genes involved in biosynthesis of valuable metabolites and degradation of toxic compounds provided information that the AFTs possess a huge library of microorganisms and genes that could be applied to further studies. All of these findings provide a significance reference for researchers working on the bacterial diversity assessment of tobacco-related samples.
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